Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100185 |
| Name | oriT_pBBR1  |
| Organism | Bordetella bronchiseptica |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_025015 (867..918 [-], 52 nt) |
| oriT length | 52 nt |
| IRs (inverted repeats) | IR1: 3..14, 18..31 (TCACGACTTTGC..GCAAAGTCTAGTGA) IR2: 36..42, 43..50 (ACTCAAG..CATTGAGT) |
| Location of nic site | 32..33 |
| Conserved sequence flanking the nic site |
TAGTGAG|TAT |
| Note | _ |
oriT sequence
Download Length: 52 nt
>oriT_pBBR1
GGTCACGACTTTGCGAAGCAAAGTCTAGTGAGTATACTCAAGCATTGAGTGG
GGTCACGACTTTGCGAAGCAAAGTCTAGTGAGTATACTCAAGCATTGAGTGG
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Szpirer CY et al. (2001) Mobilization function of the pBHR1 plasmid, a derivative of the broad-host-range plasmid pBBR1. J Bacteriol. 183(6):2101-10. [PMID:11222611]
Relaxase
| ID | 178 | GenBank | YP_009062863 |
| Name | Mob_pBBR1 |
UniProt ID | Q9Z5R6 |
| Length | 329 a.a. | PDB ID | |
| Note | involved in mobilization | ||
Relaxase protein sequence
Download Length: 329 a.a. Molecular weight: 36707.68 Da Isoelectric Point: 10.3259
>YP_009062863.1 Mob (plasmid) [Bordetella bronchiseptica]
MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHWAASSTDEAMGRLRELLPEKRRKDAV
LAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLS
AKEFIGNKAQMTRDQTTFAAAVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAP
QGLAEKLGISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALG
PLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRDRGGYSR
MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHWAASSTDEAMGRLRELLPEKRRKDAV
LAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLS
AKEFIGNKAQMTRDQTTFAAAVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAP
QGLAEKLGISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALG
PLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRDRGGYSR
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q9Z5R6 |
Reference
[1] Szpirer CY et al. (2001) Mobilization function of the pBHR1 plasmid, a derivative of the broad-host-range plasmid pBBR1. J Bacteriol. 183(6):2101-10. [PMID:11222611]
Host bacterium
| ID | 178 | GenBank | NC_025015 |
| Plasmid name | pBBR1 | Incompatibility group | - |
| Plasmid size | 2687 bp | Coordinate of oriT [Strand] | 867..918 [-] |
| Host baterium | Bordetella bronchiseptica |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |