Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100184
Name   oriT_pVA380-1 in_silico
Organism   Streptococcus sp.
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   M96957 ( , 36 nt)
oriT length   36 nt
IRs (inverted repeats)      2..8, 12..18  (CCTTTAC..GTAAAGG)
Location of nic site      26..27
Conserved sequence flanking the
  nic site    		  TAGTACG|TTA
Note   _

  oriT sequence  


Download         Length: 36 nt

>oriT_pVA380-1
ACCTTTACTATGTAAAGGCTAGTACGTTACACTTTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Grohmann E et al. (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]


Relaxase

ID   177 GenBank   AAA19677
Name   Mob_pVA380 insolico UniProt ID   Q54160
Length   430 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 430 a.a.        Molecular weight: 50913.38 Da        Isoelectric Point: 5.8467

>AAA19677.1 putative, partial [Streptococcus ferus]
MSYMVARMQKMKAGNLGGAFKHNERVFETHSNKDIDPSRSHLNYELTDRDRSVSYEKQIKDYVNENKVSN
TRAIRKDAVLCDEWIITSDKQFFEQLDEEQTRAFFETAKNYFAENYGESNIAYASVHLDESTPHMHMGVV
PFENGKLSSKSMFDREELKHIQEDLPRYMSDHGFELERGKLNSEAKHKTVAEFKRAMADMELKEELLEKY
HAPLFVDERTGELNNDTEAFWHEKEFADMFEVQSPIRETTNREKMDWLRKQYQEELKKLESSKKPLEDDL
SHLEELLDEKTKEYIKIDSETSERLSELSKAEGYINTLENYSKSLEAKIERLEREGLKLEKLKTQIADLK
IMSEKELAAITPKKGVFGKEYVELTKEQFEEFKGLIYRSRNLVHQKELENEQLRRIVPLRRSKRFEASWN
ELKKKVRERA

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-194)


  Protein structure


Source ID Structure
AlphaFold DB Q54160

  Reference


[1] LeBlanc DJ et al. (1993) Identification and characterization of a mobilization gene in the streptococcal plasmid, pVA380-1. Plasmid. 30(3):296-302. [PMID:8302939]


Host bacterium

ID   177 GenBank   M96957
Plasmid name   pVA380-1 Incompatibility group   -
Plasmid size   2476 bp Coordinate of oriT [Strand]   _
Host baterium   Streptococcus sp.

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -