Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100174 |
| Name | oriT_pIP421  |
| Organism | B.fragilis |
| Sequence Completeness | incomplete |
| NCBI accession of oriT (coordinates [strand]) | Y10480 (513..519 [+], 7 nt) |
| oriT length | 7 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | The oriT region was determined experimentally, but the putative nic site was not determined experimentally. |
oriT sequence
Download Length: 7 nt
>oriT_pIP421
CAAGCTC
CAAGCTC
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Trinh S et al. (1997) Identification and DNA sequence of the mobilization region of the 5-nitroimidazole resistance plasmid pIP421 from Bacteroides fragilis. J Bacteriol. 179(12):4071-4. [PMID:9190830]
Relaxase
| ID | 167 | GenBank | CAA71505 |
| Name | Mob421_pIP421  |
UniProt ID | P96200 |
| Length | 425 a.a. | PDB ID | |
| Note | A member of the Bacteroides mobilization protein family, which includes the MobA of pBI143, NBUs, and Tn4555 | ||
Relaxase protein sequence
Download Length: 425 a.a. Molecular weight: 49267.97 Da Isoelectric Point: 9.4676
>CAA71505.1 Mob421 protein (plasmid) [Bacteroides fragilis]
MAKTSINVRPCNIGSAERHNLRSKELDYIRPELSHRNEQWSEMKIADVLEDIREKYRQHTGQSMQKKATP
IREGVVVIEDGTTLRQLQDFAGRLEERFGIHTFQIYTHKDERASVWDGNAETWKPNYHAHMIFDWTDGET
GRTRKLNKQDMAEMQTILAECLGMERGISSDRKLLSAIQYKNMVEAEKAAQIEKECSQMEDERNAIEEKV
KQAGQKLEDTQKKLKEAAAEIKVEKLKGAAADTGTALLKAGTTVIDVTRSLFNSGKVKRQEQEIAELKRE
NYELEMKSQNLQSNLRTANAQNEREREATRRAVRTGEERLKPITNLFPCMENAKENIEELREMGVRDNDI
RLLLTGKEITYSGFLYDRERRKKHQVKNVKINIAKSKAGNTTIWLNDFHFKEFFKQLWQKLQKVLGIDKG
RGFHL
MAKTSINVRPCNIGSAERHNLRSKELDYIRPELSHRNEQWSEMKIADVLEDIREKYRQHTGQSMQKKATP
IREGVVVIEDGTTLRQLQDFAGRLEERFGIHTFQIYTHKDERASVWDGNAETWKPNYHAHMIFDWTDGET
GRTRKLNKQDMAEMQTILAECLGMERGISSDRKLLSAIQYKNMVEAEKAAQIEKECSQMEDERNAIEEKV
KQAGQKLEDTQKKLKEAAAEIKVEKLKGAAADTGTALLKAGTTVIDVTRSLFNSGKVKRQEQEIAELKRE
NYELEMKSQNLQSNLRTANAQNEREREATRRAVRTGEERLKPITNLFPCMENAKENIEELREMGVRDNDI
RLLLTGKEITYSGFLYDRERRKKHQVKNVKINIAKSKAGNTTIWLNDFHFKEFFKQLWQKLQKVLGIDKG
RGFHL
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | P96200 |
Reference
[1] Trinh S et al. (1997) Identification and DNA sequence of the mobilization region of the 5-nitroimidazole resistance plasmid pIP421 from Bacteroides fragilis. J Bacteriol. 179(12):4071-4. [PMID:9190830]
Host bacterium
| ID | 167 | GenBank | Y10480 |
| Plasmid name | pIP421 | Incompatibility group | - |
| Plasmid size | 1861 bp | Coordinate of oriT [Strand] | 513..519 [+] |
| Host baterium | B.fragilis |
Cargo genes
| Drug resistance gene | 5-nitroimidazole |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |