Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100172 |
| Name | oriT_pSa  |
| Organism | Escherichia coli |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | EU419764 (558..884 [-], 327 nt) |
| oriT length | 327 nt |
| IRs (inverted repeats) | IR1: 105..112, 120..127 (AAGTCATT..AATGACTT) IR2: 131..136, 141..146 (CGCACC..GGTGCG) IR3: 246..255, 259..268 (CCCAATGCGC..GCGCATTGGG) |
| Location of nic site | 154..155 |
| Conserved sequence flanking the nic site |
TGTCT|ATAGC |
| Note | _ |
oriT sequence
Download Length: 327 nt
>oriT_pSa
CCTCTCCTGTAGTGTTTCCGTAGTGGTTCAATCCTAGCATTTACAAGGCTTTCCGCCAATATTGTAGTAGTCGAACACTACAGCGGTTTTCGTCCTTTGCGTGGAAGTCATTGTAAATCAATGACTTACGCGCACCGAAAGGTGCGTATTGTCTATAGCCCAGATTTAAGGATACCAACCCTGCTTTTAAGGACGAAAACCATGCCGATAACGCCAGCGTGACCCTAAAGAGGGTCAAAACTGCTCCCAATGCGCTATGCGCATTGGGTTATCGCGCAACAATGATGCACTTATAATGCTATGATAGTGCTACGATGATGCAGAAAA
CCTCTCCTGTAGTGTTTCCGTAGTGGTTCAATCCTAGCATTTACAAGGCTTTCCGCCAATATTGTAGTAGTCGAACACTACAGCGGTTTTCGTCCTTTGCGTGGAAGTCATTGTAAATCAATGACTTACGCGCACCGAAAGGTGCGTATTGTCTATAGCCCAGATTTAAGGATACCAACCCTGCTTTTAAGGACGAAAACCATGCCGATAACGCCAGCGTGACCCTAAAGAGGGTCAAAACTGCTCCCAATGCGCTATGCGCATTGGGTTATCGCGCAACAATGATGCACTTATAATGCTATGATAGTGCTACGATGATGCAGAAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Revilla C et al. (2008) Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids. Antimicrob Agents Chemother. 52(4):1472-80. [PMID:18268088]
Auxiliary protein
| ID | 237 | GenBank | ABZ82007 |
| Name | TrwA_pSa |
UniProt ID | Q04229 |
| Length | 121 a.a. | PDB ID | _ |
| Note | _ | ||
Auxiliary protein sequence
Download Length: 121 a.a. Molecular weight: 13400.32 Da Isoelectric Point: 4.8464
>ABZ82007.1 TrwA (plasmid) [Escherichia coli]
MALGDPIQVRLSPEKQALLEDEAARKGKRLATYLRELLESENDLQGELAALRREVVSLHHVIEDLADTGL
RSDQSGPGQNAVQIETLLLLRAIAGPERMKPVKGELKRLGIEVWTPEGKED
MALGDPIQVRLSPEKQALLEDEAARKGKRLATYLRELLESENDLQGELAALRREVVSLHHVIEDLADTGL
RSDQSGPGQNAVQIETLLLLRAIAGPERMKPVKGELKRLGIEVWTPEGKED
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q04229 |
Reference
[1] Revilla C et al. (2008) Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids. Antimicrob Agents Chemother. 52(4):1472-80. [PMID:18268088]
Host bacterium
| ID | 165 | GenBank | EU419764 |
| Plasmid name | pSa | Incompatibility group | IncW |
| Plasmid size | 1628 bp | Coordinate of oriT [Strand] | 558..884 [-] |
| Host baterium | Escherichia coli |
Cargo genes
| Drug resistance gene | resistance to chloramphenicol, gentamicin, kanamycin, streptomycin, spectinomycin and sulfonamide |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |