Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100171
Name   oriT_pWES-1 in_silico
Organism   Salmonella enterica subsp. enterica serovar Westhampton
Sequence Completeness      incomplete
NCBI accession of oriT (coordinates [strand])   DQ268764 (2659..2670 [+], 12 nt)
oriT length   12 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   similar to Bacteroides fragilis cLV25 chromosomal transfer factor nic site; putative nic site of oriT

  oriT sequence  


Download         Length: 12 nt

>oriT_pWES-1
AGCAAGGTGTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Doublet B et al. (2009) Novel plasmid-encoded ceftazidime-hydrolyzing CTX-M-53 extended-spectrum beta-lactamase from Salmonella enterica serotypes Westhampton and Senftenberg. Antimicrob Agents Chemother. 53(5):1944-51. [PMID:19273683]


Relaxase

ID   164 GenBank   ACJ65155
Name   Orf2_pWES-1 insolico UniProt ID   B7NZZ4
Length   813 a.a. PDB ID   
Note   Relaxase/mobilization nuclease topoisomerase/primase fusion protein

  Relaxase protein sequence


Download         Length: 813 a.a.        Molecular weight: 88312.75 Da        Isoelectric Point: 9.8763

>ACJ65155.1 relaxase/mobilization nuclease topoisomerase/primase fusion protein (plasmid) [Salmonella enterica subsp. enterica serovar Westhampton]
MKAKVSRGGGFRGALNYVFDVGKEATHTKNAERVGGNMAGNDPRELSREFSAVRQLRPDIGKPVWHCSLS
LPPGERLSAEKWEAVAADFMQRMGFDQTNTPWVAVRHQDTDKDHIHIVASRVGLDGKVWLGQWEARRAIE
ATQELEHTHGLTLTPGLGDARAERRKLTDKEINMAVRTGDEPPRQRLQRLLDEAVKDKPTALELAERLQA
AGVGVRANLASTGRMNGFSFEVAGVPFKGSDLGKGYTWAGLQKAGVTYDEARDRAGLERFRPTVADRGER
QDVAAVREPDARGLEAPTGRSLDRDGADLGTAGPTPAGRDAGSGSLRQGDGHSAQDAGRADAADERERGA
GLRAEGREAGRDHLRPVAQPVRAENEPQQHGADRAAGGDLAGQAGERTAGHDESRRPTDRGSERDAPAPL
AAGAGADSGRGRGRDAGSDWASRFKQASAAKRRAADGRLGQRDLEQGHAHGARVAETDRQSARELDPTAY
LEASGYTVKREGRHLSVRAGGDEAYRVTRQQDGRWLWCDRYGNDGGDNIDLVREIEPGTGYAEAVYRLSG
APTVRQQPRPSEPKRQPPQLPAQGLAAREHGRDYLKGRGISQDTIEHAEKAGMVRYADGGVLFVGYDRAG
TAQNATRRAIAPADPVQKRDLRGSDKSYPPILPGDPAKVWIVEGGPDALALHDIAKRSGQQPPTVIVSGG
ANVRSFLERADVQAILKRAERVTVAGENEKNPEAQAKADAGHQKQAQRVAKITGREVRQWTPKPEHGKDL
ADMNARQVAEIERKRQAEIEAERARNRELSRKSRRYDGPSFGR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(11-248)


  Protein structure


Source ID Structure
AlphaFold DB B7NZZ4

  Reference


[1] Doublet B et al. (2009) Novel plasmid-encoded ceftazidime-hydrolyzing CTX-M-53 extended-spectrum beta-lactamase from Salmonella enterica serotypes Westhampton and Senftenberg. Antimicrob Agents Chemother. 53(5):1944-51. [PMID:19273683]


Auxiliary protein

ID   236 GenBank   ACJ65154
Name   MobC_pWES-1 insolico UniProt ID   B7NZZ3
Length   128 a.a. PDB ID   _
Note   auxiliary mobilization protein C

  Auxiliary protein sequence


Download         Length: 128 a.a.        Molecular weight: 14197.83 Da        Isoelectric Point: 6.4923

>ACJ65154.1 auxiliary mobilization protein C (plasmid) [Salmonella enterica subsp. enterica serovar Westhampton]
MSTDESKRRRRGPAPLDATDKRGHTVSVRLNDAELARLDSQRDAVQMQRGEYLRAAALHRLPPTIPEVNR
EAWAALARTAANLNQIAHRLNAGDALPLAEVRATLDAFRRDLIGVKHDAEDVEDESEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB B7NZZ3

  Reference


[1] Doublet B et al. (2009) Novel plasmid-encoded ceftazidime-hydrolyzing CTX-M-53 extended-spectrum beta-lactamase from Salmonella enterica serotypes Westhampton and Senftenberg. Antimicrob Agents Chemother. 53(5):1944-51. [PMID:19273683]


Host bacterium

ID   164 GenBank   DQ268764
Plasmid name   pWES-1 Incompatibility group   Col
Plasmid size   10908 bp Coordinate of oriT [Strand]   2659..2670 [+]
Host baterium   Salmonella enterica subsp. enterica serovar Westhampton

Cargo genes

Drug resistance gene   CTX-M-53 beta-lactamase encoded by blaCTX-M-53 gene; resistance to
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -