Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   100166
Name   oriT_oriT_pKL1 experimental
Organism   Escherichia coli
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_002145 (_)
oriT length   100 nt
IRs (inverted repeats)      7..15, 17..25  (GTCGGGGGT..AGCCCTGAC)
  32..50, 51..69  (GTAATCGTATCGGCGTGCA..TGCGCGGTTATACGATTAC)
Location of nic site      77..78
Conserved sequence flanking the
  nic site  
 
 CATCCTG|T
Note   _

  oriT sequence  


Download         Length: 100 nt

>oriT_oriT_pKL1
CGGGGTGTCGGGGTGAAGCCCTGACCAAGTGGTAATCGTATCGGCGTGCATGCGCGGTTATACGATTACACATCCTGTCCCGATTTCTGAGGCGTTTTAA

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Srivastava P et al. (2006) Characterization of broad host range cryptic plasmid pCR1 from Corynebacterium renale. Plasmid. 56(1):24-34. [PMID:16545871]
[2] Burian et al. (1999) Replication Control of a Small Cryptic Plasmid of Escherichia coli. J Mol Biol. 294(1):49-65. [PMID:10556028]


Host bacterium


ID   160 GenBank   NC_002145
Plasmid name   pKL1 Incompatibility group   Col
Plasmid size   1549 bp Coordinate of oriT [Strand]   334..400 [+]
Host baterium   Escherichia coli

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -