Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100161 |
| Name | oriT_pTX14-2  |
| Organism | Bacillus thuringiensis |
| Sequence Completeness | incomplete |
| NCBI accession of oriT (coordinates [strand]) | NC_004334 (2104..2127 [+], 24 nt) |
| oriT length | 24 nt |
| IRs (inverted repeats) | 1..9, 16..24 (TTTGGTATA..TATACCAAA) |
| Location of nic site | 14..15 |
| Conserved sequence flanking the nic site |
TAGTAGG|CTAT |
| Note | pMV158 superfamily |
oriT sequence
Download Length: 24 nt
>oriT_pTX14-2
TTTGGTATAGTAGGCTATACCAAA
TTTGGTATAGTAGGCTATACCAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Andrup L et al. (2003) The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes. Plasmid. 49(3):205-32. [PMID:12749835]
Relaxase
| ID | 155 | GenBank | NP_705748 |
| Name | Mob14-2_pTX14-2 |
UniProt ID | Q8GLD7 |
| Length | 445 a.a. | PDB ID | |
| Note | relaxase | ||
Relaxase protein sequence
Download Length: 445 a.a. Molecular weight: 52816.16 Da Isoelectric Point: 9.7587
>NP_705748.1 Mob14-2 (plasmid) [Bacillus thuringiensis]
MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNYDLLNQQKIRYESTIKQEISERVKRK
PRANSVVLSEFVVTASPDYMHSLSLEEQKRYFESSLAFIQKRYGKQNTLYAMVHMDEATPHMHIGVMPIT
EDNRLSAKDMFMRKELISLQQDFPLEMREKGFDVERGEGSEKKHLSPQAFKEKQDLEVEVEQLSNVKTHL
KTKVVETHNQLQQTTNYIEKQNETLQKIQQQFLSLDKKIKEKKQEFETFRNQIPDKPVSMSYLREETKTE
VTTKLFGKPEITEKKTGNIVVTREQWRDMTEKVNAAVIVKKDYERLQKTDLVKENQSLREDNKYLEETIE
GNNLALKHSYKQNRKLEEVNKELHTEIGTLKAHIRDLQMNIKVLYQQTKKVFKEQFKAFRGLIKNELDMK
GVDNQFEREHTREIRSRQKGYDMER
MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNYDLLNQQKIRYESTIKQEISERVKRK
PRANSVVLSEFVVTASPDYMHSLSLEEQKRYFESSLAFIQKRYGKQNTLYAMVHMDEATPHMHIGVMPIT
EDNRLSAKDMFMRKELISLQQDFPLEMREKGFDVERGEGSEKKHLSPQAFKEKQDLEVEVEQLSNVKTHL
KTKVVETHNQLQQTTNYIEKQNETLQKIQQQFLSLDKKIKEKKQEFETFRNQIPDKPVSMSYLREETKTE
VTTKLFGKPEITEKKTGNIVVTREQWRDMTEKVNAAVIVKKDYERLQKTDLVKENQSLREDNKYLEETIE
GNNLALKHSYKQNRKLEEVNKELHTEIGTLKAHIRDLQMNIKVLYQQTKKVFKEQFKAFRGLIKNELDMK
GVDNQFEREHTREIRSRQKGYDMER
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q8GLD7 |
Reference
[1] Andrup L et al. (2003) The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes. Plasmid. 49(3):205-32. [PMID:12749835]
Host bacterium
| ID | 155 | GenBank | NC_004334 |
| Plasmid name | pTX14-2 | Incompatibility group | - |
| Plasmid size | 6829 bp | Coordinate of oriT [Strand] | 2104..2127 [+] |
| Host baterium | Bacillus thuringiensis |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |