Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100160 |
| Name | oriT_pGI1  |
| Organism | Bacillus thuringiensis serovar thuringiensis |
| Sequence Completeness | incomplete |
| NCBI accession of oriT (coordinates [strand]) | NC_004335 (6421..6446 [+], 26 nt) |
| oriT length | 26 nt |
| IRs (inverted repeats) | 1..10, 17..26 (GTAAACTGTA..TACAGTTTAC) |
| Location of nic site | 15..16 |
| Conserved sequence flanking the nic site |
TAGTGTG|CTA |
| Note | pMV158 superfamily |
oriT sequence
Download Length: 26 nt
>oriT_pGI1
GTAAACTGTAGTGTGCTACAGTTTAC
GTAAACTGTAGTGTGCTACAGTTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Andrup L et al. (2003) The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes. Plasmid. 49(3):205-32. [PMID:12749835]
Relaxase
| ID | 154 | GenBank | NP_705753 |
| Name | Mob1_pGI1 |
UniProt ID | Q8GLD2 |
| Length | 401 a.a. | PDB ID | |
| Note | relaxase | ||
Relaxase protein sequence
Download Length: 401 a.a. Molecular weight: 47546.29 Da Isoelectric Point: 9.9007
>NP_705753.1 Mob1 (plasmid) [Bacillus thuringiensis serovar thuringiensis]
MSKFMVCHMQKFKMTDVKGLQIHNQREKESHSNSDIIQERTEQNYDLIHDKRKVDYKKIIQNRIDNGVVS
NRAIRKDAVVCCSFMISASPDYMNFLSPVEQRRFFEESVCFLKARYGEENFVYASVHVDEKTPHMHVGMV
PVNEKQKLSAYSFFKNKSELHDLQDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVTLQQEIEKLEQEK
KEIDSRLYDLKFSLNQAKSVDEIPVKEKGGFIRSKTVEIDSEDFESIKVLAKSSEVLRSENRRLKNEKIK
IEREKDDLYKGQRFLERQVTDLKRENRGLKEANDFLKKTLERVKEMYKEKLPELAGMIGYVKGSILDKMN
RKFLKRHFAGDDEVRGAQKFLNHKQEHEEQQKRLKQVRRSQQKNRDQGLER
MSKFMVCHMQKFKMTDVKGLQIHNQREKESHSNSDIIQERTEQNYDLIHDKRKVDYKKIIQNRIDNGVVS
NRAIRKDAVVCCSFMISASPDYMNFLSPVEQRRFFEESVCFLKARYGEENFVYASVHVDEKTPHMHVGMV
PVNEKQKLSAYSFFKNKSELHDLQDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVTLQQEIEKLEQEK
KEIDSRLYDLKFSLNQAKSVDEIPVKEKGGFIRSKTVEIDSEDFESIKVLAKSSEVLRSENRRLKNEKIK
IEREKDDLYKGQRFLERQVTDLKRENRGLKEANDFLKKTLERVKEMYKEKLPELAGMIGYVKGSILDKMN
RKFLKRHFAGDDEVRGAQKFLNHKQEHEEQQKRLKQVRRSQQKNRDQGLER
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q8GLD2 |
Reference
[1] Andrup L et al. (2003) The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes. Plasmid. 49(3):205-32. [PMID:12749835]
Host bacterium
| ID | 154 | GenBank | NC_004335 |
| Plasmid name | pGI1 | Incompatibility group | - |
| Plasmid size | 8254 bp | Coordinate of oriT [Strand] | 6421..6446 [+] |
| Host baterium | Bacillus thuringiensis serovar thuringiensis |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |