Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100158
Name   oriT_pSMQ172 in_silico
Organism   Streptococcus thermophilus
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   AF295100 (1332..1373 [+], 42 nt)
oriT length   42 nt
IRs (inverted repeats)      3..10, 14..20  (ACATTTAC..GTAAAGT)
Location of nic site      28..29
Conserved sequence flanking the
  nic site    		  TAGTGCG|TTAT
Note   pMV158 superfamily; the conserved recombination site (RSA): AAGTATAGTGCG|TTATACT

  oriT sequence  


Download         Length: 42 nt

>oriT_pSMQ172
GCACATTTACGAAGTAAAGTATAGTGCGTTATACTTTACATG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Turgeon N et al. (2001) Isolation and characterization of a Streptococcus thermophilus plasmid closely related to the pMV158 family. Plasmid. 45(3):171-83. [PMID:11407913]


Relaxase

ID   152 GenBank   AAK83121
Name   Mob_pSMQ172 insolico UniProt ID   Q93PW6
Length   499 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 499 a.a.        Molecular weight: 58304.84 Da        Isoelectric Point: 5.6177

>AAK83121.1 putative MOB protein (plasmid) [Streptococcus thermophilus]
MSYMVARMQKMKAGNLGGAYKHNERVFETHSNKDIDPSRSPLNYELTDRDHSVSYEKQIKDYVNENKISN
RAIRKDAVLCDEWIITSDKSFFEKLSEGETREFFETAKNYFAENYGEDNIAYASVHLDESTPHMHMGVVP
FQDGKLSSKAMFDKEELKKIQEDLPKYMNEHGFELERGELNSEAKHKTVAEFKQEMAGKEIEKQLVLEYG
APEYVNHQEEFVTKEEYQEAYEVFQNELGAWVDKSDFSWRETTFQEKIDWVKNHQQEELQQLVSSRKPLE
DEIRTLNEVLKEKYEELDKIELRASESLSELSGVEGYINNLEKHSNALESKIGGLENEYLRLAKQNARLS
DLKIMSEKELAEIQPKKGMFGKEHVELTKEQFEKFKGLIYRSKNLVHEKELENDQLRRQMPLRSSKNGFE
ASLQWVKDKTKGESIDRLKSEIRALKNENSVLRQQNDKMLGKLREFMPDKALKNFVSELKAIQPIVKIVK
RVIEKGLGL

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-194)


  Protein structure


Source ID Structure
AlphaFold DB Q93PW6

  Reference


[1] Turgeon N et al. (2001) Isolation and characterization of a Streptococcus thermophilus plasmid closely related to the pMV158 family. Plasmid. 45(3):171-83. [PMID:11407913]


Host bacterium

ID   152 GenBank   AF295100
Plasmid name   pSMQ172 Incompatibility group   -
Plasmid size   4230 bp Coordinate of oriT [Strand]   1332..1373 [+]
Host baterium   Streptococcus thermophilus

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -