Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100157
• Name: oriT_pSRQ900
• Organism: Lactococcus lactis
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_004959 (9771..10187 [+], 417 nt)
• oriT length: 417 nt
• IRs (inverted repeats): IR1: 91..100, 105..114 (CCCATTTTAT..ATAAAATGGG) IR2: 160..172, 176..188 (AAAAGGGGAAAGT..ACTTCCCTCTTTT) IR3: 195..208, 221..234 (ACATTGTAATACAA..TTGTATTACAATGT)
• Location of nic site: 243..244
• Conserved sequence flanking the nic site: CTTG|CA
• Note: similar to lactococcal plasmids transfer origins (pGL3, pGL4, pCD4, pSRQ800 and pIL7)

oriT sequence

• Length: 417 nt

>oriT_pSRQ900
CATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGACGAAATTCGTCTCTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATAAAAATAAAATGGGTAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCTCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATACTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATTTTAAAAAAAAACTATTTATAAGTTCGAAATATGTATCACAACTTTAATACGTG

oriT secondary structure

Predicted by RNAfold.

Reference

[1] Boucher I et al. (2001) DNA sequence analysis of three Lactococcus lactis plasmids encoding phage resistance mechanisms. J Dairy Sci. 84(7):1610-20. [PMID:11467810]

Host bacterium

• ID: 151
• GenBank: NC_004959
• Plasmid name: pSRQ900
• Incompatibility group: _
• Plasmid size: 10836 bp
• Coordinate of oriT [Strand]: 9771..10187 [+]
• Host baterium: Lactococcus lactis

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -