Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100156
• Name: oriT_pSRQ800
• Organism: Lactococcus lactis
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_004960 (6826..7240 [+], 415 nt)
• oriT length: 415 nt
• IRs (inverted repeats): IR1: 91..100, 105..114 (CCCATTTTAT..ATAAAATGGG) IR2: 160..172, 176..188 (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: 195..208, 221..234 (ACATTGTAATACAA..TTGTATTACAATGT)
• Location of nic site: 243..244
• Conserved sequence flanking the nic site: CTTG|CA
• Note: similar to lactococcal plasmids transfer origins (pGL3, pGL4, pCD4, pSRQ900 and pIL7)

oriT sequence

• Length: 415 nt

>oriT_pSRQ800
CATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGGAGGTGGCGTTTGCCTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTACTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATAGTTGCTATTTTGTTATGAGGATTGTAACCGAATAGGGCGCAATACTTATTACAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGTAAGTTATTGATGGATCAGAAAAATGTATCACAAATTGAAACAAAG

oriT secondary structure

Predicted by RNAfold.

Reference

[1] Boucher I et al. (2001) DNA sequence analysis of three Lactococcus lactis plasmids encoding phage resistance mechanisms. J Dairy Sci. 84(7):1610-20. [PMID:11467810]

Host bacterium

• ID: 150
• GenBank: NC_004960
• Plasmid name: pSRQ800
• Incompatibility group: _
• Plasmid size: 7858 bp
• Coordinate of oriT [Strand]: 6826..7240 [+]
• Host baterium: Lactococcus lactis

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrID1