Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100151
Name   oriT_pColK-K235 in_silico
Organism   Escherichia coli
Sequence Completeness      incomplete
NCBI accession of oriT (coordinates [strand])   NC_006881 (5471..5503 [-], 33 nt)
oriT length   33 nt
IRs (inverted repeats)     _
Location of nic site      25..26
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   ColE1 superfamily

  oriT sequence  


Download         Length: 33 nt

>oriT_pColK-K235
GTAGCGATAGCGGAGTGTATACTGGCTTAATCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Rijavec M et al. (2007) Prevalence of ColE1-like plasmids and colicin K production among uropathogenic Escherichia coli strains and quantification of inhibitory activity of colicin K. Appl Environ Microbiol. 73(3):1029-32. [PMID:17122402]


Relaxase

ID   145 GenBank   YP_214176
Name   MbkA_pColK-K235 insolico UniProt ID   Q58P29
Length   532 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 532 a.a.        Molecular weight: 59070.58 Da        Isoelectric Point: 8.5611

>YP_214176.1 MbkA (plasmid) [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGARVLRGAPEEVRELIDATPFAKKYTSGVLSFAEQTLPPGER
ERVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELASGKRLQPYYDRADRPRINAWQTLVN
HHYGLHDPNAPENRRTLVTPNNLPKAKQEAAEAITRGLEALYHAGALKTRQDVTEALTAAGFEVVRTTRS
SISIADPEGGRNLRLKGALYEQSFTNGDRLREETERACRLYREHAEQRVQEARSVCRQGTELKRAENQRR
YQRTHSSGERTTGDAEQCRERTVQRHRERTGPAAGRAALEAGQRADTVPVADGALRHGRDFSGVMGADSV
AGKPDCGKHPGYQGPGRDAGETPGKNMGCDVSRGQEREISGAAVRGEGGKQLDGGEEERSKTGQGVSEYD
RAGNALTERLRAIAAGLYAAAERLGEPLHGFAAYVRADTTGERATEGTVRRLESAGAEISRAGGPLESVI
YHEQQIRDEQERQARLERERARSWHEEQEREARVWRGPSIGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(58-239)


  Protein structure


Source ID Structure
AlphaFold DB Q58P29

  Reference


[1] Rijavec M et al. (2007) Prevalence of ColE1-like plasmids and colicin K production among uropathogenic Escherichia coli strains and quantification of inhibitory activity of colicin K. Appl Environ Microbiol. 73(3):1029-32. [PMID:17122402]


Auxiliary protein

ID   210 GenBank   YP_214177
Name   MbkB_pColK-K235 insolico UniProt ID   Q58P28
Length   186 a.a. PDB ID   _
Note   MbeB-like, N-term conserved region; pfam04837

  Auxiliary protein sequence


Download         Length: 186 a.a.        Molecular weight: 21055.07 Da        Isoelectric Point: 9.5081

>YP_214177.1 MbkB (plasmid) [Escherichia coli]
MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDAISAHTAAVKEQLETLNSAV
NAQFSATENELARRQEELLWKLVKGRILYPSLTALCVTGGIFLASWGLIQWQESRIAGNILAIRDQEETL
AKLREKTWGVTYHEGRNGKFLVLPSGVKGENNWTVEKKNAVRLVRE

  Protein domains


Predicted by InterproScan.

MbeB-like, N-term conserved region (MbeB_N)

(1-52)


  Protein structure


Source ID Structure
AlphaFold DB Q58P28

  Reference


[1] Rijavec M et al. (2007) Prevalence of ColE1-like plasmids and colicin K production among uropathogenic Escherichia coli strains and quantification of inhibitory activity of colicin K. Appl Environ Microbiol. 73(3):1029-32. [PMID:17122402]

ID   211 GenBank   YP_214175
Name   MbkC_pColK-K235 insolico UniProt ID   Q58P30
Length   67 a.a. PDB ID   _
Note   mobilization protein

  Auxiliary protein sequence


Download         Length: 67 a.a.        Molecular weight: 8093.61 Da        Isoelectric Point: 12.0190

>YP_214175.1 MbkC (plasmid) [Escherichia coli]
MIPVKRERMLTIRVTDEEHARLLARCEGKQLASWMRKVCLGHRHRKRQVFRHWHRRCCASSLRSAIT

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB Q58P30

  Reference


[1] Rijavec M et al. (2007) Prevalence of ColE1-like plasmids and colicin K production among uropathogenic Escherichia coli strains and quantification of inhibitory activity of colicin K. Appl Environ Microbiol. 73(3):1029-32. [PMID:17122402]


Host bacterium

ID   145 GenBank   NC_006881
Plasmid name   pColK-K235 Incompatibility group   ColRNAI
Plasmid size   8318 bp Coordinate of oriT [Strand]   5471..5503 [-]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -