Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100149
Name   oriT_pHH1107 in_silico
Organism   Uncultured bacterium HH1107
Sequence Completeness      incomplete
NCBI accession of oriT (coordinates [strand])   NC_019299 (26636..26643 [-], 8 nt)
oriT length   8 nt
IRs (inverted repeats)     _
Location of nic site      7..8
Conserved sequence flanking the
  nic site    		  TATCCTG|C
Note   

  oriT sequence  


Download         Length: 8 nt

>oriT_pHH1107
TATCCTGC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   143 GenBank   YP_006962113
Name   MobC_pHH1107 insolico UniProt ID   B5TTL0
Length   219 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 219 a.a.        Molecular weight: 25662.49 Da        Isoelectric Point: 8.7079

>YP_006962113.1 MobC (plasmid) [uncultured bacterium HH1107]
MTKDNKIFFRVDRAVLDWWEAKCEKQGIKKGAPFQKALELSYQKENDPEYSGQDFSLNLEKDLAEKIYHL
AKFEEVDPDDLIKKMIKEKLSEIEKTQDVIDVFEKTSRKELRLKPSLMKAINEKAAQIGMKPNQYIISVL
TANVTKDSIFFSQEEALKLGESNSQLLAIGRNLNQMAKNMNQGIYESYDREFVERVHQLVKAQVRHVFTL
LERNKKRWE

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB B5TTL0


T4CP

ID   143 GenBank   YP_006962092
Name   TraN_pHH1107 insolico UniProt ID   B5TTI9
Length   707 a.a. PDB ID   _
Note   coupling of DNA transfer and mating pair formation

  T4CP protein sequence


Download         Length: 707 a.a.        Molecular weight: 78880.56 Da        Isoelectric Point: 4.1633

>YP_006962092.1 TraN (plasmid) [uncultured bacterium HH1107]
MGNKGILVGKYHNKYLMLGGQQFVLLAAPTRSGKGVAIVIPNLLNYSDSVVVLDLKLENFLLTSKFRAKN
GQKVYLFSPFSEDLKSHKWNPFDTVSHDENFRVGEILAIGRSFYPVTGDAKTDFWNDNANNLFLGLALYL
FETEDLPVTMGEVLRQSSGKGKPIQDHIRSIIDERAQSDKPLSDTCLDALNRFLSSPDNTLGNIISTFTS
PLTIFANPIVDAATSSSDFKLEDVRRQRMSIYFGVQPNRLGDAKNLINLFYSLLIDVNLKTLPQQDSSLK
YQCLVVNDEATAVGKVAKIADANSFIAGYNIRLLTIIQSIGQLEDVYKTQTRGLVTNHALQIVYPPREQR
DANEYSEMLGYFTFKAKSKGQSRSMAWGQGGSNSENESDQRRALMLPQELKELSQDKEIIFMENTKPIMC
EKAKYFNDSAFVDRLKSVSPSMKAIKGFPTHKQLEKIAFEDLELSIPIPDLDVKLHKAKIEKRIAAITTV
EQAEQIDISSLVIPEFQLDKTVFEDPENPQPEECLQVVDQFFAAIEWEEDTEAQNIVDDFFSQVEASAEN
PVESLVAVGASEQAENLVAADDSDPLAMIAEMKNLIAVDEPEQAENLVAADESDPLAMIAEMENLIAVDE
PEQLENLISVDDTEFSYDEMDAEQIVDTFFNCAESEFSIDEDLQDNPVAADEGQNGSIENSDLDDVYQAF
SNQVNSK

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(9-441)

  Protein structure


Source ID Structure
AlphaFold DB B5TTI9


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2505..15332

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ST1107_03 100..486 - 387 YP_006962089 hypothetical protein -
ST1107_04 913..2202 - 1290 YP_006962090 TraO -
ST1107_05 2213..2494 - 282 YP_006962091 hypothetical protein -
ST1107_06 2505..4628 - 2124 YP_006962092 TraN tfc24
ST1107_07 4958..6031 - 1074 YP_006962093 TraM virB11
ST1107_08 6051..7475 - 1425 YP_006962094 TraL virB10
ST1107_09 7475..8365 - 891 YP_006962095 TraK virB9
ST1107_10 8377..9192 - 816 YP_006962096 TraJ virB8
ST1107_11 9355..10056 - 702 YP_006962097 TraF virB5
ST1107_12 10068..11093 - 1026 YP_006962098 TraH virB6
ST1107_13 11117..11416 - 300 YP_006962099 hypothetical protein -
ST1107_14 11419..13881 - 2463 YP_006962100 TraE virb4
ST1107_15 13889..14215 - 327 YP_006962101 TraD virB3
ST1107_16 14215..14520 - 306 YP_006962102 TraC virB2
ST1107_17 14655..15332 - 678 YP_006962103 TraA virB1
ST1107_18 15332..17584 - 2253 YP_006962104 TraB -
ST1107_19 17868..18146 - 279 YP_006962105 hypothetical protein -
ST1107_20 18964..19284 - 321 YP_006962106 hypothetical protein -

Host bacterium

ID   143 GenBank   NC_019299
Plasmid name   pHH1107 Incompatibility group   -
Plasmid size   57951 bp Coordinate of oriT [Strand]   26636..26643 [-]
Host baterium   Uncultured bacterium HH1107

Cargo genes

Drug resistance gene   tetracycline resistance
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -