Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100148 |
| Name | oriT_pSMA23  |
| Organism | Lacticaseibacillus casei |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NC_010242 (529..556 [+], 28 nt) |
| oriT length | 28 nt |
| IRs (inverted repeats) | 3..12, 20..29 (GTAAAGTATA..TATACCTTAC) |
| Location of nic site | 16..17 |
| Conserved sequence flanking the nic site |
TATTGG|GCTAT |
| Note | pMV158 superfamily; nick site (TGG|G) |
oriT sequence
Download Length: 28 nt
>oriT_pSMA23
AGGTAAAGTATATTGGGCTATACCTTAC
AGGTAAAGTATATTGGGCTATACCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Sudhamani M et al. (2008) Characterisation of pSMA23, a 3.5 kbp plasmid of Lactobacillus casei, and application for heterologous expression in Lactobacillus. Plasmid. 59(1):11-9. [PMID:17961648]
Relaxase
| ID | 142 | GenBank | YP_001649176 |
| Name | Mob_pSMA23 |
UniProt ID | Q45TE0 |
| Length | 364 a.a. | PDB ID | |
| Note | clade A of the pMV158-superfamily of relaxases | ||
Relaxase protein sequence
Download Length: 364 a.a. Molecular weight: 42121.57 Da Isoelectric Point: 9.7310
>YP_001649176.1 mobilization protein (plasmid) [Lactobacillus casei]
MSYLVANMQKLKADNLVGLGNHDQRRTQHHKNPDIDVDRSALNYDLVAGRTGHFKKTDIEAYINEHKTSQ
RAVRKDAVLVNEWIISSDSHFFADLTAADMRKYFETAKDYFAEKFGEENIRYAIVHLDESTPHMHMGIVP
FDDEHKLSAKRVFNRTALRDVQDQLPAYLQQHGFNIQRGVQESERKSLTVPEYKAMRESIKQSQQKLAAV
ENETKQRQAKLKTYQATKFDVNSVKTKESRFHKRYVLVDRFDFDKLKQGASLTDTYFTETLSQRSDMDIQ
KDQLIKAESKAMELDIENRRLQKLVGTLQGIVRSVDRFLQRKLGVGLPSEWLERAGLKEPSKKAPQRPQE
RSEGQHDKLDGPSL
MSYLVANMQKLKADNLVGLGNHDQRRTQHHKNPDIDVDRSALNYDLVAGRTGHFKKTDIEAYINEHKTSQ
RAVRKDAVLVNEWIISSDSHFFADLTAADMRKYFETAKDYFAEKFGEENIRYAIVHLDESTPHMHMGIVP
FDDEHKLSAKRVFNRTALRDVQDQLPAYLQQHGFNIQRGVQESERKSLTVPEYKAMRESIKQSQQKLAAV
ENETKQRQAKLKTYQATKFDVNSVKTKESRFHKRYVLVDRFDFDKLKQGASLTDTYFTETLSQRSDMDIQ
KDQLIKAESKAMELDIENRRLQKLVGTLQGIVRSVDRFLQRKLGVGLPSEWLERAGLKEPSKKAPQRPQE
RSEGQHDKLDGPSL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q45TE0 |
Reference
[1] Sudhamani M et al. (2008) Characterisation of pSMA23, a 3.5 kbp plasmid of Lactobacillus casei, and application for heterologous expression in Lactobacillus. Plasmid. 59(1):11-9. [PMID:17961648]
Host bacterium
| ID | 142 | GenBank | NC_010242 |
| Plasmid name | pSMA23 | Incompatibility group | - |
| Plasmid size | 3497 bp | Coordinate of oriT [Strand] | 529..556 [+] |
| Host baterium | Lacticaseibacillus casei |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |