Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100147 |
| Name | oriT_pH205  |
| Organism | Klebsiella pneumoniae |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_010261 (7094..7311 [-], 218 nt) |
| oriT length | 218 nt |
| IRs (inverted repeats) | IR1: 46..53, 57..64 (TTCGGGGC..GCCCTGAA) IR2: 77..80, 81..84 (CGCT..AGCG) |
| Location of nic site | 97..98 |
| Conserved sequence flanking the nic site |
CTGG|CTAA |
| Note | ColE1 superfamily |
oriT sequence
Download Length: 218 nt
>oriT_pH205
GATGGCATGGGGTGATATCCAGTAGCGGAGAGGTTGTTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTAAATATGTTACGGCGGAGTGGTGATTTATGAAAGTGCTTCATGTGACAAGGGAAAAAATGCGGCTACCGGGCGTAAGTGCAACATGCAGGTAAGGATGAAATGACGCCTCCTCGCTCAC
GATGGCATGGGGTGATATCCAGTAGCGGAGAGGTTGTTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTAAATATGTTACGGCGGAGTGGTGATTTATGAAAGTGCTTCATGTGACAAGGGAAAAAATGCGGCTACCGGGCGTAAGTGCAACATGCAGGTAAGGATGAAATGACGCCTCCTCGCTCAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Zioga A et al. (2009) CMY-31 and CMY-36 cephalosporinases encoded by ColE1-like plasmids. Antimicrob Agents Chemother. 53(3):1256-9. [PMID:19104021]
Relaxase
| ID | 141 | GenBank | YP_001649335 |
| Name | MobB_pH205 |
UniProt ID | B0FJX9 |
| Length | 156 a.a. | PDB ID | |
| Note | relaxase | ||
Relaxase protein sequence
Download Length: 156 a.a. Molecular weight: 17556.39 Da Isoelectric Point: 9.3491
>YP_001649335.1 relaxase (plasmid) [Klebsiella pneumoniae]
MQQAIRMRIEADSASGKMTETVRQSVNAALTQVEKELKQTGLEQQKPATDAFNQAAGTAKAMIHEMRREM
SRYTWKSAIYIAMTMIAVMGGCLWGMYYFIDSGYARVAEMQHMEAVWQKKAPLADIKTCDGNPCVKVDIS
RTFGDKKDTYLIIKGK
MQQAIRMRIEADSASGKMTETVRQSVNAALTQVEKELKQTGLEQQKPATDAFNQAAGTAKAMIHEMRREM
SRYTWKSAIYIAMTMIAVMGGCLWGMYYFIDSGYARVAEMQHMEAVWQKKAPLADIKTCDGNPCVKVDIS
RTFGDKKDTYLIIKGK
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | B0FJX9 |
Reference
[1] Zioga A et al. (2009) CMY-31 and CMY-36 cephalosporinases encoded by ColE1-like plasmids. Antimicrob Agents Chemother. 53(3):1256-9. [PMID:19104021]
Host bacterium
| ID | 141 | GenBank | NC_010261 |
| Plasmid name | pH205 | Incompatibility group | _ |
| Plasmid size | 8197 bp | Coordinate of oriT [Strand] | 7094..7311 [-] |
| Host baterium | Klebsiella pneumoniae |
Cargo genes
| Drug resistance gene | CMY-31 cephalosporinase (blaCMY-type gene) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |