Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100145
• Name: oriT_pCT14
• Organism: Pseudomonas sp. CT14
• Sequence Completeness: incomplete
• NCBI accession of oriT (coordinates [strand]): NC_010891 (17343..17372 [-], 30 nt)
• oriT length: 30 nt
• IRs (inverted repeats): _
• Location of nic site: 14..15
• Conserved sequence flanking the nic site: GGTGT|ATAGC
• Note: _

oriT sequence

• Length: 30 nt

>oriT_pCT14
ATTGCGTATGGTGTATAGCTCGTTAAGGGA

oriT secondary structure

Predicted by RNAfold.

Reference

[1] Bramucci M et al. (2006) Genetic organization of a plasmid from an industrial wastewater bioreactor. Appl Microbiol Biotechnol. 71(1):67-74. [PMID:16244860]

Relaxase

TraI_pCT14

• ID: 139
• GenBank: YP_001966297
• Name: TraI_pCT14
• UniProt ID: Q2QCQ9
• Length: 984 a.a.
• Note: relaxase

Relaxase protein sequence

• Length: 984 a.a.
• Molecular weight: 108500.49 Da
• Isoelectric Point: 9.7219

>YP_001966297.1 TraI (plasmid) [Pseudomonas sp. CT14]
MLDITTISRQSLGKVVSYYADGADDYYAKDGGAMQWQGAGAEALGLSGEVEQARFRELLDGRISDSTKLM
RTVKEADGKVVSKERLGYDLTFSAPKGVSLQALVHGDASIIEAHDKAVAAAIREAERLSQARITVNKKTG
TENTNNLVVAKFRHETSRALDPDLHTHAFVLNMTQRSDGEWRALKNDGVFNSSMFLGNVYKAELARELEK
AGFQLRYERNGTFDLAHFSDEQIREFSSRSQQIEAALAAKGLDRSTASYAEKNQAALATRDKKQGGIDRE
ELRQVWLERSRALGIDYHSREWAGVGADAQGGRERNSAATPQIEKPLEYRADQVIEFAIKSLTERQAVIG
QKELMDTALRHGYGALTIDDVRAGIERRAASGHLIREEPLYSSQNPADGKKGKAAEKAREEAPQLSRKEW
VATLVRAGKSRSEAARLVDEGIRTGRLRQGENRFTTHIAQKREREVLQIERMGRGTVEPRISKEAAEAFL
ADRGLKAEQQASVMRIARTQNQFIGVQGFAGVGKSYMTVAAKDLLEANGYRVTSLAPYGSQVKALQAEGL
EARTLQSFLKARDKKIDSNTVVFIDEAGVIPARQMHEAMKTIEAAGARVVFLGDVAQTKAIEAGKPFEQL
MKAGMETSRLTDIQRQKDPQLLEAVKLAAEGKAKQSLPLVNEIREIKEDGARYQAIVDAYAKMTKAERDQ
ALIITGTNASRIQINEGVREALGLKGQGAEYPLLNRLDTTQAERRHSKYYGKGSIVVPEVDYKNGLQRGV
QYVVLDTGPGNKLTVRGPEGETLQFTPARCTKLSVYSVERTELVAGDQVKITRNDAEKDLANGDRFVVKE
IHSDRVVLEGDKGRQVELDTASAMFVGLAYASTVHSAQGLTCDKVLINLETQSRTTAKDVYYVAISRARH
AAEIFTDNRQKLGDAISRLNAKAGALDIKQLQRHALERKGHDQAGKQKDAVQKQQQGQQLPAKQPKEKGR
AFGL

Protein domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C_2) (869-911)

• TrwC relaxase (TrwC) (14-292)

• AAA domain (AAA_30) (495-676)

Protein structure

• AlphaFold DB: Q2QCQ9

Reference

[1] Bramucci M et al. (2006) Genetic organization of a plasmid from an industrial wastewater bioreactor. Appl Microbiol Biotechnol. 71(1):67-74. [PMID:16244860]

T4CP

TraJ_pCT14

• ID: 139
• GenBank: YP_001966296
• Name: TraJ_pCT14
• UniProt ID: Q2QCR0
• Length: 512 a.a.
• PDB ID: _
• Note: Type IV secretion-system coupling protein

T4CP protein sequence

• Length: 512 a.a.
• Molecular weight: 58134.81 Da
• Isoelectric Point: 8.8819

>YP_001966296.1 TraJ (plasmid) [Pseudomonas sp. CT14]
MNERQRSQICLAVMVLVPLFAWFFTAKLMYGIEKQGARDRVFYLAKNTFTLWPLAVALVGGLILAIALCV
LIVKLSKTTFAGAHFDKYFRGSQLVSQRELKRLTKEKEEQITIAGVPVPIAAEATHFSVGGATGTGKSTI
FREMMYGCMQRKDRMVILDPDGEFLSTFYRKGDKILNPYDARTEGWNFYNEIRSHYDFERYAKSIIQVSD
SNDSEEWNQYGRLLFAEVAKKLYNTTRNPGMREVFRWTNECDSETLQAFVKGTRAVSLFTENERATGSVR
FVLSNKLPAHFDMPPGNFSLRQWCEDPNGGNLFITWDENMREALRPLISCWVDTIFTSILGMKSNPKRRI
WTFLDELESLQRLPTLGDMLTRGRKKGGCVVSGYQSYTQLESVYGEKLAETMLANHRTMVALAVGRMGTA
TAERMSKALSEHEVLRTKEGRSSRWGDWGTRSENEDVKPERIVMSSEIMALNNLEGFLSFPGSIPIARVT
IDPINFTRTNPVPGIVPNLEII

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (111-491)

Protein structure

• AlphaFold DB: Q2QCR0

Reference

[1] Bramucci M et al. (2006) Genetic organization of a plasmid from an industrial wastewater bioreactor. Appl Microbiol Biotechnol. 71(1):67-74. [PMID:16244860]

Host bacterium

• ID: 139
• GenBank: NC_010891
• Plasmid name: pCT14
• Incompatibility group: IncN-like
• Plasmid size: 55216 bp
• Coordinate of oriT [Strand]: 17343..17372 [-]
• Host baterium: Pseudomonas sp. CT14

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: mercury resistance
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -