Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100143 |
| Name | oriT_pMG2200  |
| Organism | Enterococcus faecalis |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_011642 (43622..43943 [+], 322 nt) |
| oriT length | 322 nt |
| IRs (inverted repeats) | IR1: 224..231, 233..240 (GCCTTGCA..TGCAAGGC) IR2: 241..247, 253..259 (GGGTCAG..CTGACCC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | _ |
oriT sequence
Download Length: 322 nt
AGAACAATCAACAACTAATTAGGCAAATTAAGGGGTGCTAAACAACTGCTAGTAGGTGCTAGAGATGTGCTATAAAGGGTGCTAGTTTGGTGCTAGTTACTGCTAAATACGTGCTAGTTTAGGTGCTAGAAACGTGCTATATGGTGCTAAAAAGGTGCTAGTTTGCATGAAGTTACCTGCTAGCCAAGTGCTAGTGGCGTTCGTTTTTGGGTCCCACGGGAAAGCCTTGCACTGCAAGGCGGGTCAGCTTGTCTGACCCCCATTTCCCCTTATGCTCTTCCGAAACACAAAGCGCAATTAAGCGAATACTAGAGAATAAATA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Zheng B et al. (2009) Isolation of VanB-type Enterococcus faecalis strains from nosocomial infections: first report of the isolation and identification of the pheromone-responsive plasmids pMG2200, Encoding VanB-type vancomycin resistance and a Bac41-type bacteriocin, and pMG2201, encoding erythromycin resistance and cytolysin (Hly/Bac). Antimicrob Agents Chemother. 53(2):735-47. [PMID:19029325]
Relaxase
| ID | 137 | GenBank | YP_002333406 |
| Name | ORF44_pMG2200 |
UniProt ID | B6ZHK5 |
| Length | 686 a.a. | PDB ID | |
| Note | putative relaxase (plasmid) | ||
Relaxase protein sequence
Download Length: 686 a.a. Molecular weight: 80619.53 Da Isoelectric Point: 5.5455
MNKKTAGIILTSAFVMPGTDTFKEYLDYMERSEATRNEHFDQFNAFSPDNSMDSIAAVEGSNETMFSTYN
DYMSNPKKTSALFTNNYDQAPEEVIRYMKEYFEMAQENKSPLWQLVFSFRNEWLIENNYLDPETNQLKTQ
HIYQATRLAIAELEKKEGLKGEWTGAVHYNTDNIHVHVGYVEKNPTREWIFYKHPKEPSKTGWQFKGKFL
QKSIKATKRTFVNELMQNKEQLSMLETYVTHQKKLAESCKEQFTQGKYEQLFTDLLQKLPSNQYFWKYGF
AERHKFKPELDKIVDLFMETDGKETMDEILPRLQKISDDYEEAYGNPRNEPTYLDKQLYGKSGLYSRIGN
MILQEAKNYPELLAKRKTGLMNESDFEQFNFETEIESEGLIEPFDSLEMTMPQEENAEESMIYDDVLPHE
LLEESHEEVNNIEEQIKVKQSENKMDQALKTLHDYFTEREESKDSRNLKIVDQLKAEKESSKKEIRGMFL
HEVEGNQFAEPVSSFTHETLGRKIILPSKNKAHYISIETSKDKEVDSNLQSFSGNNQRAILQQIPTATHV
LGEKQWQLNNRQIREDEIENAIKIVTIKKDESGSFVFETKDVYDISQTDKLPKKERNANYKIEKATEKNG
QKRVTKVSNRKPVVSHKKSDYEFKKQLRQLRKSCERSVQRYLNEKAYQELEQSMNI
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | B6ZHK5 |
Reference
[1] Zheng B et al. (2009) Isolation of VanB-type Enterococcus faecalis strains from nosocomial infections: first report of the isolation and identification of the pheromone-responsive plasmids pMG2200, Encoding VanB-type vancomycin resistance and a Bac41-type bacteriocin, and pMG2201, encoding erythromycin resistance and cytolysin (Hly/Bac). Antimicrob Agents Chemother. 53(2):735-47. [PMID:19029325]
T4CP
| ID | 137 | GenBank | YP_002333362 |
| Name | VirD4_pMG2200 |
UniProt ID | B6ZHS5 |
| Length | 843 a.a. | PDB ID | _ |
| Note | coupling protein (plasmid) | ||
T4CP protein sequence
Download Length: 843 a.a. Molecular weight: 97849.39 Da Isoelectric Point: 7.8063
MKKKEKGKFKKKNYEKAKQNNLRFLADKRFLIPAMIFLFLTEIIVGNWLLQNIFSLLKSNLFSIQWVEFK
ETLFYYPLSEYLFAYILLFIASILSTSIIAYRMYTSFRSLEEEHVQGTMDFESTEGMDKQYPTVEVHPFT
KTEDFYDGKPGYPVGRKPKTMKQKAEGTFSLYVDTTDSNTITLAGTRQGKGIYFVDTFIDILTRARLIAN
RASFVMTATKGDEPRKWYNTLKRRGYRIRIANVVNPYYSDPIPALAVFNNYYRHYKRLKKESVGLKEKGK
ASERLNVSIKAEQQLSNAEKIIKQVAFSYFREVNEGKDGGFWTKACRSLFMSVGLALADQEYEENETSKV
NPYTIYNVVNDMQRRRIKENSHEFLKKYSNDENELQRLLAEYENKSELDVFFGELPRTHPAKRHYDAIFA
SAPAHVTLGNIITHFDGDLEPFLMTLNAKMTAIDDGFDMELVGFDREQPTAVFVMLSDADTSNNQLGVMY
LEQIYQVLLNRCNLEDDSQCYRDVHFIYEEGGNLGVAISDLARKWTAGLSRRLFSHLVLQDIQQLSSLYD
EDTKDIILGNTGNLAYIRTGSESTNKYISGRLGDRSNYSKTRHKDPLSIKSTETESTERIDLLASFELER
LREGESVILRINKHRDLEGNPIYQYPIYNTYENDTNLIPFYKFRKLDKVSWEEIPVNNQFMEIELEDLNW
KLQPGKIIDQQKQVIPQIPTYKKPERRKDSNSITPIEQIAKKEVKSVFSTEKYAKEAKFLEDMYGDKLEH
LVINVFSVEQQKRLIALIQMTGEKEPQSFAELERVSKTGNVRSYISCILSSFGQGAVNMVVQQLKEWRNS
NEF
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | B6ZHS5 |
Reference
[1] Zheng B et al. (2009) Isolation of VanB-type Enterococcus faecalis strains from nosocomial infections: first report of the isolation and identification of the pheromone-responsive plasmids pMG2200, Encoding VanB-type vancomycin resistance and a Bac41-type bacteriocin, and pMG2201, encoding erythromycin resistance and cytolysin (Hly/Bac). Antimicrob Agents Chemother. 53(2):735-47. [PMID:19029325]
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 6442..19228
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HXC41_RS00015 (pMG2200_03) | 2019..2399 | + | 381 | WP_002360820 | hypothetical protein | - |
| HXC41_RS00020 (pMG2200_04) | 3068..5084 | + | 2017 | Protein_3 | trse protein | - |
| HXC41_RS00025 | 5087..5275 | + | 189 | WP_002360822 | hypothetical protein | - |
| HXC41_RS00030 (pMG2200_06) | 5294..6445 | + | 1152 | WP_002382030 | DUF3991 domain-containing protein | - |
| HXC41_RS00035 (pMG2200_07) | 6442..7524 | + | 1083 | WP_012579098 | lysozyme family protein | orf14 |
| HXC41_RS00040 (pMG2200_08) | 7588..7821 | + | 234 | WP_010829130 | hypothetical protein | - |
| HXC41_RS00045 (pMG2200_09) | 7822..8148 | + | 327 | WP_002360827 | type II toxin-antitoxin system PemK/MazF family toxin | - |
| HXC41_RS00050 (pMG2200_10) | 8240..8635 | + | 396 | WP_238827655 | hypothetical protein | - |
| HXC41_RS00055 (pMG2200_11) | 8831..10030 | + | 1200 | WP_002317296 | DUF6017 domain-containing protein | - |
| HXC41_RS00060 (pMG2200_12) | 10052..10264 | + | 213 | WP_002317295 | DUF5348 domain-containing protein | - |
| HXC41_RS00065 (pMG2200_13) | 10339..10815 | + | 477 | WP_002317294 | PcfB family protein | cd411 |
| HXC41_RS00070 (pMG2200_14) | 10812..12620 | + | 1809 | WP_002317293 | VirD4-like conjugal transfer protein, CD1115 family | - |
| HXC41_RS00075 | 12697..12912 | + | 216 | WP_002317292 | Maff2 family mobile element protein | prgF |
| HXC41_RS00080 (pMG2200_15) | 13004..13867 | + | 864 | WP_002317291 | VirB6/TrbL-like conjugal transfer protein, CD1112 family | prgHa |
| HXC41_RS00085 (pMG2200_16) | 13898..14440 | + | 543 | WP_002317290 | MT-A70 family methyltransferase | - |
| HXC41_RS00090 (pMG2200_17) | 14454..14876 | + | 423 | WP_002370797 | PrgI family protein | prgIa |
| HXC41_RS00095 (pMG2200_18) | 14806..17205 | + | 2400 | WP_002317289 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| HXC41_RS00100 (pMG2200_19) | 17237..19228 | + | 1992 | WP_002382864 | C40 family peptidase | cd419a |
| HXC41_RS00105 (pMG2200_20) | 19251..19502 | + | 252 | WP_002317286 | DUF4315 family protein | - |
| HXC41_RS00110 (pMG2200_21) | 19492..20721 | + | 1230 | WP_002317285 | CD1107 family mobile element protein | - |
| HXC41_RS00115 (pMG2200_22) | 20718..22799 | + | 2082 | WP_002317284 | DNA topoisomerase 3 | - |
Host bacterium
| ID | 137 | GenBank | NC_011642 |
| Plasmid name | pMG2200 | Incompatibility group | _ |
| Plasmid size | 106527 bp | Coordinate of oriT [Strand] | 43622..43943 [+] |
| Host baterium | Enterococcus faecalis |
Cargo genes
| Drug resistance gene | vancomycin resistance |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |