Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100138
Name   oriT_pAMbeta1 in_silico
Organism   Enterococcus faecalis
Sequence Completeness      incomplete
NCBI accession of oriT (coordinates [strand])   NC_013514 (_)
oriT length   47 nt
IRs (inverted repeats)      IR1: 3..10, 12..19  (CATAGAAT..ATTCTTTG)
  IR2: 25..31, 32..38  (TTCAATC..GATTCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   _

  oriT sequence  


Download         Length: 47 nt

>oriT_pAMbeta1
GTCATAGAATAATTCTTTGTTTTCTTCAATCGATTCAATAGAAATAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Kurenbach B et al. (2003) Intergeneric transfer of the Enterococcus faecalis plasmid pIP501 to Escherichia coli and Streptomyces lividans and sequence analysis of its tra region. Plasmid. 50(1):86-93. [PMID:12826062]


Relaxase

ID   133 GenBank   YP_003305363
Name   ORF19_pAMbeta1 insolico UniProt ID   D1MAV5
Length   654 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 654 a.a.        Molecular weight: 76447.53 Da        Isoelectric Point: 9.4303

>YP_003305363.1 AM19 (plasmid) [Enterococcus faecalis]
MTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTLWNKMELA
EKQVNSQICREVNVALPIELNNTDQRSLIEEFVKDNFVSEGMIADVAIHRDDENNPHAHIMLTMREVDSE
GNILNKRKKIPKLDENGNPILLENGKIKMVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNIDQQITEK
SHAELGKKELPTIHEGFYSKKLEEKGIISELKRKNLEIQSYNDILAELDKLESQEKVLKQDQNFTLKFEK
TFSPLEKGELKNLSKELKLFISDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISSERDMLTKANE
ILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEESKKIFKEKPF
QTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDSVIEGEML
LAKADYYKTTDFSKVEGVARFSSEEVNSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKHIDELSPLAK
HNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSGNQLQKKR
NLDKLIKQTKAKKNQSLQRNIPLR

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(11-235)

Nicking enzyme C-terminal middle helical domain (NES_C_h)

(282-387)


  Protein structure


Source ID Structure
AlphaFold DB D1MAV5


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 15400..27714

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS679_RS00065 (pAMbeta1_p13) 10479..10796 + 318 WP_002333463 hypothetical protein -
HS679_RS00070 (pAMbeta1_p14) 11330..11827 + 498 WP_002325587 DnaJ domain-containing protein -
HS679_RS00075 (pAMbeta1_p15) 11861..12166 + 306 WP_002425015 hypothetical protein -
HS679_RS00080 (pAMbeta1_p16) 12189..12446 - 258 WP_002325623 hypothetical protein -
HS679_RS00085 (pAMbeta1_p17) 12449..12748 - 300 WP_002325624 hypothetical protein -
HS679_RS00090 (pAMbeta1_p18) 13040..15025 + 1986 WP_025186512 MobQ family relaxase -
HS679_RS00095 (pAMbeta1_p19) 15049..15381 + 333 WP_000713503 CagC family type IV secretion system protein -
HS679_RS00100 (pAMbeta1_p20) 15400..15783 + 384 WP_001083514 glycosyl transferase trsC
HS679_RS00105 (pAMbeta1_p21) 15800..16429 + 630 WP_025186513 hypothetical protein trsD
HS679_RS00110 (pAMbeta1_p22) 16440..18401 + 1962 WP_002425003 TrsE protein virb4
HS679_RS00115 (pAMbeta1_p23) 18415..19767 + 1353 WP_002425004 hypothetical protein trsF
HS679_RS00120 (pAMbeta1_p24) 19789..20898 + 1110 WP_002425005 lysozyme family protein orf14
HS679_RS00125 (pAMbeta1_p25) 20911..21462 + 552 WP_002425006 hypothetical protein -
HS679_RS00130 (pAMbeta1_p26) 21467..21898 + 432 WP_002425007 hypothetical protein -
HS679_RS00135 (pAMbeta1_p27) 21891..23546 + 1656 WP_002425008 VirD4-like conjugal transfer protein, CD1115 family -
HS679_RS00140 (pAMbeta1_p28) 23564..24487 + 924 WP_002325631 hypothetical protein -
HS679_RS00145 (pAMbeta1_p29) 24489..25421 + 933 WP_002325632 conjugal transfer protein TrbL family protein trsL
HS679_RS00150 (pAMbeta1_p30) 25438..26409 + 972 WP_002425009 hypothetical protein -
HS679_RS00155 (pAMbeta1_p31) 26438..26806 + 369 WP_000516429 hypothetical protein -
HS679_RS00160 (pAMbeta1_p32) 26866..27714 + 849 WP_012858058 LPXTG cell wall anchor domain-containing protein prgC

Host bacterium

ID   133 GenBank   NC_013514
Plasmid name   pAMbeta1 Incompatibility group   Inc18
Plasmid size   27815 bp Coordinate of oriT [Strand]   
Host baterium   Enterococcus faecalis

Cargo genes

Drug resistance gene   resistance to macrolide, lincosamide, streptogramin B (MLS)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -