Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100137
Name   oriT_pGNB2 in_silico
Organism   Uncultured bacterium
Sequence Completeness      incomplete
NCBI accession of oriT (coordinates [strand])   DQ460733 (_)
oriT length   12 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   _

  oriT sequence  


Download         Length: 12 nt

>oriT_pGNB2
TTACACCTTGCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Bönemann G et al. (2006) Mobilizable IncQ-related plasmid carrying a new quinolone resistance gene, qnrS2, isolated from the bacterial community of a wastewater treatment plant. Antimicrob Agents Chemother. 50(9):3075-80. [PMID:16940104]


Relaxase

ID   132 GenBank   ABE98193
Name   ORF1_pGNB2 insolico UniProt ID   Q0GY57
Length   813 a.a. PDB ID   
Note   relaxase/mobilization nuclease topoisomerase/primase fusion protein

  Relaxase protein sequence


Download         Length: 813 a.a.        Molecular weight: 88020.14 Da        Isoelectric Point: 9.1912

>ABE98193.1 relaxase/mobilization nuclease topoisomerase/primase fusion protein (plasmid) [uncultured bacterium]
MKAKVSRGGGFRGALNYVFDVGKEATHTKNAERVGGNMAGNDPRELSREFSAVRQLRPDIGKPVWHCSLS
LPPGERLSAEKWEAVTTDFMQRMGFDQTNTPWVAVRHQDTDKDHIHIVASRVGLDGKVWLGQWEARSAIE
ATQELEHTHGLTLTPGLGDARAERRKLTDKEINMAVRTGDEPPRQRLQRLLDEAVKDKPTAPELADRLHA
AGVGVRANLASTGRMNGFSFEVAGVPFKGSDLGKGYTWAGLQKSGVTYDEARDRAGLERFRPTVADRGER
QDVAAVPEPDARGLEAPTGRSLDRDGADLGTAGPTPTGRDAGSGSLRQGDGHSAQDAGRADAADERERGE
GVRAEGRGAGQDHLRPVQQPVRAGNEHQQHEADRGAGGGLAGQSGERTEGHDASRQQTDRGSERDAPAPL
AAGAGADSGRGRGRDAGSDWASRFKQASAAKRRAADGGLGQRDLEQGHAQGARVAGTDRQSARELDPTAY
LEASGYTVKREGRHLSVKAGGDEAYRVTRQQDGRWLWCDRYGNDGGDNIDLVREIEPGTGYAEAVYRLSG
APTVRQQPRPSEPKRQPPQLPAQGLAAREHGRDYLKGRGISPDTIEHAEKAGMVRYADGGVLFVGYDRTG
TAQNATRRAIAPADPVQKRDLRGSDKIYPPILPGDPAKVWIVEGGPDALALHDIAKRSGQQPPTVIVSGG
ANVRSFLERDDVQAILKRAERVTVAGENEKNPEAQAKADAGHQKQAQRVAEITGREVRQWTPKPEHGKDL
ADMNARQVAEIERKRQAEIEAERARNRELSRKSRGYDGPSFGR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(11-251)


  Protein structure


Source ID Structure
AlphaFold DB Q0GY57

  Reference


[1] Bönemann G et al. (2006) Mobilizable IncQ-related plasmid carrying a new quinolone resistance gene, qnrS2, isolated from the bacterial community of a wastewater treatment plant. Antimicrob Agents Chemother. 50(9):3075-80. [PMID:16940104]


Auxiliary protein

ID   195 GenBank   ABE98194
Name   MobC_pGNB2 insolico UniProt ID   Q0GY56
Length   143 a.a. PDB ID   _
Note   auxiliary mobilization protein C

  Auxiliary protein sequence


Download         Length: 143 a.a.        Molecular weight: 15833.73 Da        Isoelectric Point: 6.8761

>ABE98194.1 auxiliary mobilization protein C (plasmid) [uncultured bacterium]
MNTDESKRRRRGPAPLDATDKRGHTVSVRLNDAELARLDSQRDAVQMQRGEYLRAAALHRLPPTIPAANR
EQWAELARTAANLNQIARHLNEGQRGDGERIGKALGLQLSAELANCLRLLVLVRGDLIGVKHDSEDVEDE
SEG

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(75-95)


  Protein structure


Source ID Structure
AlphaFold DB Q0GY56

  Reference


[1] Bönemann G et al. (2006) Mobilizable IncQ-related plasmid carrying a new quinolone resistance gene, qnrS2, isolated from the bacterial community of a wastewater treatment plant. Antimicrob Agents Chemother. 50(9):3075-80. [PMID:16940104]


Host bacterium

ID   132 GenBank   DQ460733
Plasmid name   pGNB2 Incompatibility group   IncQ2
Plasmid size   8469 bp Coordinate of oriT [Strand]   
Host baterium   Uncultured bacterium

Cargo genes

Drug resistance gene   quinolone resistance
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -