Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100133 |
| Name | oriT_pGL3  |
| Organism | Lactococcus garvieae |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_016970 (65..297 [+], 233 nt) |
| oriT length | 233 nt |
| IRs (inverted repeats) | IR1: 46..55, 60..69 (CCCATTTTAT..ATAAAATGGG) IR2: 115..127, 131..143 (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: 150..163, 176..189 (ACATTGTAATACAA..TTGTATTACAATGT) |
| Location of nic site | 198..199 |
| Conserved sequence flanking the nic site |
CTTG|CA |
| Note | 98% DNA identical to oriT of the lactococcal plasmids pCD4, pSRQ800, pSRQ900 and pIL7 |
oriT sequence
Download Length: 233 nt
>oriT_pGL3
CTCTTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCCTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGTTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTAT
CTCTTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCCTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGTTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Aguado-Urda M et al. (2012) Characterization of plasmids in a human clinical strain of Lactococcus garvieae. PLoS One. 7(6):e40119. [PMID:22768237]
Host bacterium
| ID | 128 | GenBank | NC_016970 |
| Plasmid name | pGL3 | Incompatibility group | _ |
| Plasmid size | 12948 bp | Coordinate of oriT [Strand] | 65..297 [+] |
| Host baterium | Lactococcus garvieae |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |