Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100130
Name   oriT_pEC34B in_silico
Organism   Escherichia coli
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_019078 (2543..2630 [+], 88 nt)
oriT length   88 nt
IRs (inverted repeats)      8..17, 21..30  (GTGTCGGGGC..GCCCTGACCC)
Location of nic site      61..62
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   ColE1 superfamily

  oriT sequence  


Download         Length: 88 nt

>oriT_pEC34B
TCAGGGGGTGTCGGGGCGAAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGTGGCATCAGTGCGGATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   125 GenBank   YP_006953583
Name   MobA_pEC34B insolico UniProt ID   G9BMJ6
Length   517 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 517 a.a.        Molecular weight: 57594.05 Da        Isoelectric Point: 6.6784

>YP_006953583.1 plasmid mobilization protein (plasmid) [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRTLTLPDNLPETKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGLEVVRVTRS
SISIADPNGGKNIRLKGAFYEQSFTDGRGVREKAERESRIYRDNAERRVQQARKICKQGCDLKRDENQRR
YSPVHNVDRGIAGKTPGRGERGDDAAQEGRVKAGREYGHEVAGDSLSPVYREWRDALVSWRADTGEPGRN
QDTGRNIAETEREDMGRGVCAGREKEISGPSVREIGNGDSLSGEGLGTTDGVIQSDGAGKTFAERIRATA
TGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQEIRDERLIQE
REHALSLERERQPEIQERTLDGPSLGW

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-237)


  Protein structure


Source ID Structure
AlphaFold DB G9BMJ6


Auxiliary protein

ID   187 GenBank   YP_006953585
Name   MobB_pEC34B insolico UniProt ID   G9BMJ8
Length   172 a.a. PDB ID   _
Note   plasmid mobilization protein

  Auxiliary protein sequence


Download         Length: 172 a.a.        Molecular weight: 19458.35 Da        Isoelectric Point: 8.4191

>YP_006953585.1 plasmid mobilization protein (plasmid) [Escherichia coli]
MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNETKIKDAIALFTTSTEESLEKHREGV
KEAMMQHRKDVLKLAGNTGMRLLGIVFLLFTASGGTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEF
VQDGRRKFLVLPYGKSATVIPYQGKDWVQLTE

  Protein domains


Predicted by InterproScan.

MbeB-like, N-term conserved region (MbeB_N)

(1-52)


  Protein structure


Source ID Structure
AlphaFold DB G9BMJ8

ID   188 GenBank   YP_006953586
Name   MobC_pEC34B insolico UniProt ID   G9BMJ9
Length   115 a.a. PDB ID   _
Note   plasmid mobilization protein

  Auxiliary protein sequence


Download         Length: 115 a.a.        Molecular weight: 12994.13 Da        Isoelectric Point: 11.3039

>YP_006953586.1 plasmid mobilization protein (plasmid) [Escherichia coli]
MIPMKRERMLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTLAPPLLRQLAAIGNNLNQ
TARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHGARDDS

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(58-102)


  Protein structure


Source ID Structure
AlphaFold DB G9BMJ9


Host bacterium

ID   125 GenBank   NC_019078
Plasmid name   pEC34B Incompatibility group   ColRNAI
Plasmid size   6952 bp Coordinate of oriT [Strand]   2543..2630 [+]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -