Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100127
Name   oriT_pLJ42 in_silico
Organism   Lactiplantibacillus plantarum
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_019219 (_)
oriT length   310 nt
IRs (inverted repeats)      IR1: 1..9, 28..36  (AAAAGCCGA..TCGGCTTTT)
  IR2: 57..66, 67..76  (TGTCCTTTTTTTG..CAAAAAAAGGGCA)
  IR3: 153..164, 168..179  (AAAAGGGGGAGC..GCGCCCTCTTTT)
  IR4: 199..204, 208..213  (TTTTGC..GCAAAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   _

  oriT sequence  


Download         Length: 310 nt

>oriT_pLJ42
AAAAGCCGATTTGCGAACAGCAAACTTTCGGCTTTTTTTGTGCGGTTTTTTTGTAACCTTTTTTTGCAAAAAAAGGGCAGGTTTTTGGGTTTGAACTTGTGTCGTTTTGCGAAGCGAAATCGGCATGCTTTTGACTTTGATTTTAGGGGGATAAAAGGGGGAGCTAAGCGCCCTCTTTTCAAGCGGGAATGGGCATACTTTTGCGAAGCAAAATGTATGTCATTCCGAAAGCTTGCAGTATTTGTTTTTTAATGTTAGGGTAAACGTGACCGAAAAGGAAGCGTTTATACTTACATTAACTCATTAAAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   122 GenBank   YP_006960783
Name   MobA_pLJ42 insolico UniProt ID   Q4F8M8
Length   445 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 445 a.a.        Molecular weight: 51002.71 Da        Isoelectric Point: 9.4357

>YP_006960783.1 MobA (plasmid) [Lactobacillus plantarum]
MATIAKITNGASAASALNYALGKDKELHNDTKQWLEDNRLERPPELTNCRAVAVGGTNGIDISIANEQFK
IVQQAFNQTARRNQVLRITQSFALNELDPTNTRDWQRANDLGCELAEKLYPDYQTAVYTHLDGQNHILHN
HIIINKVNLQTGKKLDERKGSAVERARNANDEISKEQGWKVIEPVREHQSRTEQDLTKKGQYSYMADLRG
RIDTTMQDTSICDFKTFSDVLGQSGVIVRERGQSFSYAFLDANNKQRRARGKRLGTDYDKEAIDNELARR
TRERTEQQLDREDQARSTELVRDTTATDTALEQRERDTEPRKPALNQQSTKIERLGTSMQEINDRGSHAE
QIYQRIRPAVRTIADGLQRFTDAVHGFAKQIKQRLADQHLYQDVASRFKADMARKEQAQHKEVKQDLAKR
TAPKPQRAPERSTQTYYHDRGGLER

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure


Source ID Structure
AlphaFold DB Q4F8M8


Auxiliary protein

ID   183 GenBank   YP_006960784
Name   MobB_pLJ42 insolico UniProt ID   Q4F8M7
Length   229 a.a. PDB ID   _
Note   mobilization protein

  Auxiliary protein sequence


Download         Length: 229 a.a.        Molecular weight: 26099.19 Da        Isoelectric Point: 7.1851

>YP_006960784.1 MobB (plasmid) [Lactobacillus plantarum]
MSQNEELAKQILTAYANGQVDLTNVPDLQKMVKEAAAQQMAHTYSTTDFHAKQEEALAHEKAWYHQEIDR
ILQEKLTEIEPKTLKGAVTDQLKQQLADLEHQQAEFEATVERAKSTGNRQFWQSLIPVLAGTSVCLLLVA
VIFFVLEKLIYQGLWNGWGLHKLYNVVMAIQPQHPYGAIVLGILGFVLIAGAIYASFWLLVHAVQQLVDF
KPFKPSKLLFWKKQNNTRW

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB Q4F8M7

ID   184 GenBank   YP_006960782
Name   MobC_pLJ42 insolico UniProt ID   Q4F8M9
Length   112 a.a. PDB ID   _
Note   mobilization protein

  Auxiliary protein sequence


Download         Length: 112 a.a.        Molecular weight: 12944.87 Da        Isoelectric Point: 10.6780

>YP_006960782.1 MobC (plasmid) [Lactobacillus plantarum]
MANIKANSDKQTKRINFRLNELEYEKLSQSASTYGLTVSSYAKQLALKSNLRKPYFSASDTQQIILELTR
QGTNLNQITRKLNQGDPLTPAMLAEIKKMQEVQRQLWRQLQK

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(67-111)


  Protein structure


Source ID Structure
AlphaFold DB Q4F8M9


Host bacterium

ID   122 GenBank   NC_019219
Plasmid name   pLJ42 Incompatibility group   -
Plasmid size   5529 bp Coordinate of oriT [Strand]   
Host baterium   Lactiplantibacillus plantarum

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -