Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100124 |
| Name | oriT_pMBLT00  |
| Organism | UNVERIFIED: Leuconostoc mesenteroides subsp. mesenteroides strain KCTC 13302 |
| Sequence Completeness | incomplete |
| NCBI accession of oriT (coordinates [strand]) | JN106352 (17020..17039 [-], 20 nt) |
| oriT length | 20 nt |
| IRs (inverted repeats) | 1..9, 12..20 (CTCGTAAAA..TTTTTCGAG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note |
oriT sequence
Download Length: 20 nt
>oriT_pMBLT00
CTCGAAAAAGGTTTTACGAG
CTCGAAAAAGGTTTTACGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Chae HS et al. (2013) Use of a novel Escherichia coli-leuconostoc shuttle vector for metabolic engineering of Leuconostoc citreum to overproduce D-lactate. Appl Environ Microbiol. 79(5):1428-35. [PMID:23241984]
Relaxase
| ID | 119 | GenBank | AFJ91158 |
| Name | MobA_pMBLT00 |
UniProt ID | I6Q9D0 |
| Length | 309 a.a. | PDB ID | |
| Note | mobilization protein; binds to an origin of transfer (oriT). | ||
Relaxase protein sequence
Download Length: 309 a.a. Molecular weight: 35521.10 Da Isoelectric Point: 6.3756
>AFJ91158.1 mobilization protein (plasmid) [Leuconostoc mesenteroides subsp. mesenteroides]
MAHLKKNTRGAVPGLAVHFERKTDHHTNKDIDVSKTYLNQDLMTDGSDMISRFNDRLSDVYCMKRDDVKA
LATWIVTLPEELAEASYEQQEAFFEETKHFLDERYGKENTMAAVVHYDETTPHLHYAFVPVVFDAKKERY
KVSAKQVLTRHDLQTFHDDLDQDLKKVLPFYEKGVLNNKTLPFENVAEIKKYNDQFKALKDELTNVEENI
SAKKALLKITNQELDEVDLAEKQIDDFKQALSKNLFGKTVIKPDDLDRFKTVLANMKKATLQSQRNAEQT
SHELSKVKEQLADAKAEHQNLKISSATSG
MAHLKKNTRGAVPGLAVHFERKTDHHTNKDIDVSKTYLNQDLMTDGSDMISRFNDRLSDVYCMKRDDVKA
LATWIVTLPEELAEASYEQQEAFFEETKHFLDERYGKENTMAAVVHYDETTPHLHYAFVPVVFDAKKERY
KVSAKQVLTRHDLQTFHDDLDQDLKKVLPFYEKGVLNNKTLPFENVAEIKKYNDQFKALKDELTNVEENI
SAKKALLKITNQELDEVDLAEKQIDDFKQALSKNLFGKTVIKPDDLDRFKTVLANMKKATLQSQRNAEQT
SHELSKVKEQLADAKAEHQNLKISSATSG
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | I6Q9D0 |
Reference
[1] Chae HS et al. (2013) Use of a novel Escherichia coli-leuconostoc shuttle vector for metabolic engineering of Leuconostoc citreum to overproduce D-lactate. Appl Environ Microbiol. 79(5):1428-35. [PMID:23241984]
Host bacterium
| ID | 119 | GenBank | JN106352 |
| Plasmid name | pMBLT00 | Incompatibility group | - |
| Plasmid size | 20721 bp | Coordinate of oriT [Strand] | 17020..17039 [-] |
| Host baterium | UNVERIFIED: Leuconostoc mesenteroides subsp. mesenteroides strain KCTC 13302 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |