Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100123
Name   oriT_pRSB105 in_silico
Organism   Uncultured bacterium
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   DQ839391 (26859..27169 [-], 311 nt)
oriT length   311 nt
IRs (inverted repeats)      IR1:80..87, 90..97  (CAAGGGGC..GCCCCTTG)
  IR2: 155..162, 166..173  (CACGTGGC..GCCATGTG)
Location of nic site      192..193
Conserved sequence flanking the
  nic site    		  CATCCTG|T
Note   

  oriT sequence  


Download         Length: 311 nt

>oriT_pRSB105
CGTGTCTCCTTCTAGCCGGGGAGCGAGGGGGGCGGGGTGTCTTGGAAAGTAAGAAGCGAAGCGCCGCACTGTCCGGGGTCAAGGGGCTTGCCCCTTGCAGGATGAGCCAGACCGTGAGAAGCGAAGCGCCGAATGGTCTGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAACGGCCATCCTGTTAAGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGACGCACTTTGCAACAAGCACAAATGGCGCTATGCTGTCAACTCCGCAACAAGTAAGCAAAGGAGTTAGCACG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Schlüter A et al. (2007) Erythromycin resistance-conferring plasmid pRSB105, isolated from a sewage treatment plant, harbors a new macrolide resistance determinant, an integron-containing Tn402-like element, and a large region of unknown function. Appl Environ Microbiol. 73(6):1952-60. [PMID:17261525]


Relaxase

ID   118 GenBank   ABI20469
Name   MobA_pRSB105 insolico UniProt ID   A1Y2V1
Length   889 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 889 a.a.        Molecular weight: 101332.84 Da        Isoelectric Point: 9.8368

>ABI20469.1 mobilization protein (plasmid) [uncultured bacterium]
MIVKKVPNSKTKHKTGSIRDLADYIREPHNQNPDEKVLYANGRGFLCDGHAAQREEMVALAAESVRSKNP
VNHYIMSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINNG
FDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTGEKSAERIAIE
DGAPIIKKAQTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQRQ
QVAQREPEPIKPDVPGWKDYITGRKAHYAEKNAAKLALDKRQEQERKQIAEQQKARRDELMRGNWKGKGE
VLNAMRSVIAAEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEGA
SGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGSF
TVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKSEQLKQFELYAEAVGAERYRVT
SIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLRLQRRGENLYYTPLSDKKHHILIDDMNREKLE
RLIRDGYRPAVVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRK
PKHQREDGSYPEVRLLKAERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDAY
QRHYRDVIKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARYA
YSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(438-526)

Relaxase/Mobilisation nuclease domain (Relaxase)

(17-272)

RepB DNA-primase from phage plasmid (RepB_primase)

(548-806)


  Protein structure


Source ID Structure
AlphaFold DB A1Y2V1

  Reference


[1] Schlüter A et al. (2007) Erythromycin resistance-conferring plasmid pRSB105, isolated from a sewage treatment plant, harbors a new macrolide resistance determinant, an integron-containing Tn402-like element, and a large region of unknown function. Appl Environ Microbiol. 73(6):1952-60. [PMID:17261525]


Auxiliary protein

ID   177 GenBank   ABI20468
Name   MobB_pRSB105 insolico UniProt ID   A1Y2V0
Length   103 a.a. PDB ID   _
Note   MobB; plasmid mobilization

  Auxiliary protein sequence


Download         Length: 103 a.a.        Molecular weight: 11389.21 Da        Isoelectric Point: 9.9109

>ABI20468.1 mobilization protein (plasmid) [uncultured bacterium]
MPFETQGPEPLDAVINVRLTAAEKAKLKEDADLAGLSMSELVRRRYFGRPIIANADAVMLKELRRIGGLL
KHIHNESGGIYSKDTAGALLAVKAYIERLSRDR

  Protein domains


Predicted by InterproScan.

Ribbon-helix-helix protein, copG family (RHH_1)

(15-44)


  Protein structure


Source ID Structure
AlphaFold DB A1Y2V0

  Reference


[1] Schlüter A et al. (2007) Erythromycin resistance-conferring plasmid pRSB105, isolated from a sewage treatment plant, harbors a new macrolide resistance determinant, an integron-containing Tn402-like element, and a large region of unknown function. Appl Environ Microbiol. 73(6):1952-60. [PMID:17261525]

ID   178 GenBank   ABI20467
Name   MobC_pRSB105 insolico UniProt ID   A1Y2U9
Length   118 a.a. PDB ID   _
Note   MobC; plasmid mobilization

  Auxiliary protein sequence


Download         Length: 118 a.a.        Molecular weight: 13019.08 Da        Isoelectric Point: 10.4080

>ABI20467.1 mobilization protein (plasmid) [uncultured bacterium]
MKCTTEQLEAIAAKLREMPPVEKKKQEHSKQDAVRILSKEIALLQKRGYTLDQISETLRGEGLSIATPTL
KSYLQRAKPAKKAPVQAPGDTPRPRPAVKKPADTSKATFTPKPDSDDI

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A1Y2U9

  Reference


[1] Schlüter A et al. (2007) Erythromycin resistance-conferring plasmid pRSB105, isolated from a sewage treatment plant, harbors a new macrolide resistance determinant, an integron-containing Tn402-like element, and a large region of unknown function. Appl Environ Microbiol. 73(6):1952-60. [PMID:17261525]


Host bacterium

ID   118 GenBank   DQ839391
Plasmid name   pRSB105 Incompatibility group   IncP6
Plasmid size   57137 bp Coordinate of oriT [Strand]   26859..27169 [-]
Host baterium   Uncultured bacterium

Cargo genes

Drug resistance gene   erythromycin resistance
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -