Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100117
• Name: oriT_pSF301-3
• Organism: Shigella flexneri
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NC_019250 (228..260 [-], 33 nt)
• oriT length: 33 nt
• IRs (inverted repeats): _
• Location of nic site: 25..26
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: ColE1 superfamily

oriT sequence

• Length: 33 nt

>oriT_pSF301-3
GTAGCGATAGCGGAGTGTATACTGGCTTAACCA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Mob_pSF301-3

• ID: 112
• GenBank: YP_006961080
• Name: Mob_pSF301-3
• UniProt ID: F6MFW0
• Length: 537 a.a.
• Note: Relaxase; plasmid mobilization protein

Relaxase protein sequence

• Length: 537 a.a.
• Molecular weight: 60541.25 Da
• Isoelectric Point: 6.6506

>YP_006961080.1 plasmid mobilization protein (plasmid) [Shigella flexneri]
MSCAGCVWPSGNRGRGMIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYT
SGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYY
DRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAESITRWIDALYHVGEIKGRQDVI
QALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFTDGRGVREKAERESRIYRENAERRVQEARR
ICKQGCDIKRDENQRRYSPVHSFDRGITEKTSGRGERGDDAAQEGRVKAGREYGHDVTGDGHFPVYREWR
DALVSWRDDTGEPGRNQEAERESGEAEREDMGRALSRGEQRTFSGVARGDESRNELDDGKRETECRKAGT
GVTEHDGAGKPFAERIRAAATGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADR
TLEPVIQRELEIREERLIQEREHALFLERERQPEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (73-253)

Protein structure

• AlphaFold DB: F6MFW0

Host bacterium

• ID: 112
• GenBank: NC_019250
• Plasmid name: pSF301-3
• Incompatibility group: ColRNAI
• Plasmid size: 4043 bp
• Coordinate of oriT [Strand]: 228..260 [-]
• Host baterium: Shigella flexneri

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -