Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100115 |
| Name | oriT_pU302S  |
| Organism | Salmonella enterica subsp. enterica serovar Typhimurium |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_006815 (1211..1451 [-], 241 nt) |
| oriT length | 241 nt |
| IRs (inverted repeats) | IR1: 20..27, 31..38 (GTCGGGGC..GCCCTGAC) IR2: 45..62, 65..82 (AATTGTAATAGCGTGCAT..ATGCGCGGTATAACAATT) IR3: 150..160, 179..189nt (TGTTGTCTGAC..GTCAGACAGCA) |
| Location of nic site | 92..93 |
| Conserved sequence flanking the nic site |
CATCCTG|T |
| Note | ColE1 superfamily |
oriT sequence
Download Length: 241 nt
>oriT_pU302S
CTCCGTATCTGACATGGGTGTCGGGGCAAAGCCCTGACCAGGGTAATTGTAATAGCGTGCATGTATGCGCGGTATAACAATTGCACATCCTGTCCTGTCAGCAAGCTCGAATTACCGACAAAAGCACGGCAGTTTTTTGGCAGTGACAGTGTTGTCTGACAGAAATGCCCGTCAAAGAGTCAGACAGCAGAATACCGGCGGTAAAACAGTCGAAAAGTGGTACTCGATATAATCACACAAC
CTCCGTATCTGACATGGGTGTCGGGGCAAAGCCCTGACCAGGGTAATTGTAATAGCGTGCATGTATGCGCGGTATAACAATTGCACATCCTGTCCTGTCAGCAAGCTCGAATTACCGACAAAAGCACGGCAGTTTTTTGGCAGTGACAGTGTTGTCTGACAGAAATGCCCGTCAAAGAGTCAGACAGCAGAATACCGGCGGTAAAACAGTCGAAAAGTGGTACTCGATATAATCACACAAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Chen CY et al. (2007) Complete nucleotide sequences of 84.5- and 3.2-kb plasmids in the multi-antibiotic resistant Salmonella enterica serovar Typhimurium U302 strain G8430. Plasmid. 57(1):29-43. [PMID:16828159]
Auxiliary protein
| ID | 166 | GenBank | YP_194806 |
| Name | MobA_pU302S |
UniProt ID | Q5QJQ5 |
| Length | 107 a.a. | PDB ID | _ |
| Note | conjugal transfer relaxosome component TraJ | ||
Auxiliary protein sequence
Download Length: 107 a.a. Molecular weight: 12073.05 Da Isoelectric Point: 11.0390
>YP_194806.1 plasmid mobilization protein A (plasmid) [Salmonella enterica subsp. enterica serovar Typhimurium]
MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRAAALGKKVNSLTDDRVLKEVMRLGAL
QKKLFIDGKRVGDREYAEVLIAITEYHRALLSRLMAD
MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRAAALGKKVNSLTDDRVLKEVMRLGAL
QKKLFIDGKRVGDREYAEVLIAITEYHRALLSRLMAD
Protein domains
No domain identified.
Protein structure
No available structure.
Host bacterium
| ID | 110 | GenBank | NC_006815 |
| Plasmid name | pU302S | Incompatibility group | ColRNAI |
| Plasmid size | 3208 bp | Coordinate of oriT [Strand] | 1211..1451 [-] |
| Host baterium | Salmonella enterica subsp. enterica serovar Typhimurium |
Cargo genes
| Drug resistance gene | aph(3')-Ia |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |