Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100111
Name   oriT_Rms149 in_silico
Organism   Pseudomonas aeruginosa
Sequence Completeness      incomplete
NCBI accession of oriT (coordinates [strand])   NC_007100 (33033..33049 [+], 17 nt)
oriT length   17 nt
IRs (inverted repeats)     _
Location of nic site      13..14
Conserved sequence flanking the
  nic site    		  CATCCTG|T
Note   by analogy with pTF-FC2, pTC-F14, direction inferred from R751

  oriT sequence  


Download         Length: 17 nt

>oriT_Rms149
AACGGCCATCCTGTTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Haines AS et al. (2005) The IncP-6 plasmid Rms149 consists of a small mobilizable backbone with multiple large insertions. J Bacteriol. 187(14):4728-38. [PMID:15995187]


Relaxase

ID   106 GenBank   YP_245456
Name   MobA_Rms149 insolico UniProt ID   Q4W1T4
Length   601 a.a. PDB ID   
Note   MobA mobilisation protein

  Relaxase protein sequence


Download         Length: 601 a.a.        Molecular weight: 68654.23 Da        Isoelectric Point: 9.2818

>YP_245456.1 MobA mobilisation protein (plasmid) [Pseudomonas aeruginosa]
MADYIREPHNQNPDEKVLYANGRGFLCDGHAAQREEMVALAAESVRSKNPVNHYIMSWREGEQPSPEQVE
EAVSIFMDELGVKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINNGFDIEAAHKAIARIENAQGWQ
REQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTGEKSAERIAIEDGAPIIKKAQTWEQLHRELA
AKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQRQQVAQREPEPIKPDVPGWKDY
ITGRKAHYAAKNAAKLALDKQQEQERKQLAEQQKARRDELMRGNWNGKGEVLNAMRSVIAAEQAAEKAAL
KEKHKKQREQWRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEGASGEPPTPRDIRAYQPEIVGQ
QVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGSFTVTGNDEYKAMCAKLAAEHG
FKITNPELQERIQQERQRIQQERAQAMKSEQLKQFELYAEAVGAERYRVTSIKMQADGRKQTFILDKKDG
ITRGFTPQEIEQRTPEMLRLAQLRLLDHLIAFHLTNQLHSI

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(16-252)

Large polyvalent protein-associated domain 7 (LPD7)

(418-506)


  Protein structure


Source ID Structure
AlphaFold DB Q4W1T4

  Reference


[1] Haines AS et al. (2005) The IncP-6 plasmid Rms149 consists of a small mobilizable backbone with multiple large insertions. J Bacteriol. 187(14):4728-38. [PMID:15995187]


Auxiliary protein

ID   160 GenBank   YP_245458
Name   MobB_Rms149 insolico UniProt ID   Q4W1T3
Length   103 a.a. PDB ID   _
Note   MobB mobilisation protein

  Auxiliary protein sequence


Download         Length: 103 a.a.        Molecular weight: 11389.21 Da        Isoelectric Point: 9.9109

>YP_245458.1 MobB mobilisation protein (plasmid) [Pseudomonas aeruginosa]
MPFETQGPEPLDAVINVRLTAAEKAKLKEDADLAGLSMSELVRRRYFGRPIIANADAVMLKELRRIGGLL
KHIHNESGGIYSKDTAGALLAVKAYIERLSRDR

  Protein domains


Predicted by InterproScan.

Ribbon-helix-helix protein, copG family (RHH_1)

(15-44)


  Protein structure


Source ID Structure
AlphaFold DB Q4W1T3

ID   161 GenBank   YP_245459
Name   MobC_Rms149 insolico UniProt ID   Q4W1T2
Length   118 a.a. PDB ID   _
Note   MobC mobilisation protein

  Auxiliary protein sequence


Download         Length: 118 a.a.        Molecular weight: 13038.02 Da        Isoelectric Point: 10.3326

>YP_245459.1 MobC mobilisation protein (plasmid) [Pseudomonas aeruginosa]
MKYTTEQLEAIASKLREMPPVEKKKQEHSKQEAVRVLSKEIAALQKRGYTLDQISETLRGEGLSIATPTL
KSYLQRAKPTKKAPVQAPGDTPPPRPAIKKPADTSKATFTPKPDSDDI

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB Q4W1T2


Host bacterium

ID   106 GenBank   NC_007100
Plasmid name   Rms149 Incompatibility group   IncP6
Plasmid size   57121 bp Coordinate of oriT [Strand]   33033..33049 [+]
Host baterium   Pseudomonas aeruginosa

Cargo genes

Drug resistance gene   sul1, qacE, aadA5, aac(3)-I
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -