Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100109 |
| Name | oriT_pST12  |
| Organism | Salmonella enterica subsp. enterica serovar Derby str. T12 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_022376 (5989..6230 [-], 242 nt) |
| oriT length | 242 nt |
| IRs (inverted repeats) | 22..29, 33..40 (GTCGGGGC..GCCCTGAC) |
| Location of nic site | 94..95 |
| Conserved sequence flanking the nic site |
CATCCTG|T |
| Note | ColE1 superfamily |
oriT sequence
Download Length: 242 nt
>oriT_pST12
GCTCCGTATCTGACATGGGGTGTCGGGGCGAAGCCCTGACCAGGGTAATTGTAATAGCGTGCATGTATGCGCGGTATAACAATTGCACATCCTGTCCTGTCAGCAAGCTCGAATTACCGACAAAGGCACGGCAGTTTTTTGGCAGTGACAGTATTGTCAGGCAGAAATACCCGTCAAAGCGTCAGACGGCAGAATACCGGCGGTAAAACAGTCGAAAAGTGGTACGCGATATAATCACACAA
GCTCCGTATCTGACATGGGGTGTCGGGGCGAAGCCCTGACCAGGGTAATTGTAATAGCGTGCATGTATGCGCGGTATAACAATTGCACATCCTGTCCTGTCAGCAAGCTCGAATTACCGACAAAGGCACGGCAGTTTTTTGGCAGTGACAGTATTGTCAGGCAGAAATACCCGTCAAAGCGTCAGACGGCAGAATACCGGCGGTAAAACAGTCGAAAAGTGGTACGCGATATAATCACACAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Vignoli R et al. (2006) New TEM-Derived Extended-Spectrum 尾-Lactamase and Its Genomic Context in Plasmids from Salmonella enterica Serovar Derby Isolates from Uruguay. Antimicrob Agents Chemother. 50(2):781-4. [PMID:16436745]
Relaxase
| ID | 104 | GenBank | YP_008574978 |
| Name | MobA_pST12 |
UniProt ID | T2MZH7 |
| Length | 145 a.a. | PDB ID | |
| Note | plasmid mobilization protein | ||
Relaxase protein sequence
Download Length: 145 a.a. Molecular weight: 16419.13 Da Isoelectric Point: 10.6379
>YP_008574978.1 plasmid mobilization protein (plasmid) [Salmonella enterica subsp. enterica serovar Derby str. T12]
MLTGQDVQLLYRAYMHAITITLVRASPRHPMSDTEPCFMTKRSGSNTRRRAISRPVRLTAEEDQEIRKRA
AECGKTVSGFLRAAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLS
RLMAD
MLTGQDVQLLYRAYMHAITITLVRASPRHPMSDTEPCFMTKRSGSNTRRRAISRPVRLTAEEDQEIRKRA
AECGKTVSGFLRAAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLS
RLMAD
Protein domains
No domain identified.
Protein structure
No available structure.
Reference
[1] Vignoli R et al. (2006) New TEM-Derived Extended-Spectrum 尾-Lactamase and Its Genomic Context in Plasmids from Salmonella enterica Serovar Derby Isolates from Uruguay. Antimicrob Agents Chemother. 50(2):781-4. [PMID:16436745]
Host bacterium
| ID | 104 | GenBank | NC_022376 |
| Plasmid name | pST12 | Incompatibility group | ColRNAI |
| Plasmid size | 8275 bp | Coordinate of oriT [Strand] | 5989..6230 [-] |
| Host baterium | Salmonella enterica subsp. enterica serovar Derby str. T12 |
Cargo genes
| Drug resistance gene | blaTEM-144 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |