Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100107 |
| Name | oriT_pMBUI1  |
| Organism | Uncultured bacterium |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | JQ432563 (32348..32390 [+], 43 nt) |
| oriT length | 43 nt |
| IRs (inverted repeats) | 1..19, 22..40 (GTGAAGATAGTTAACCGGC..GCCGGTTAGCTAACTTCAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note |
oriT sequence
Download Length: 43 nt
>oriT_pMBUI1
GTGAAGATAGTTAACCGGCTTGCCGGTTAGCTAACTTCACCTA
GTGAAGATAGTTAACCGGCTTGCCGGTTAGCTAACTTCACCTA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 102 | GenBank | AFS51493 |
| Name | TraI_pMBUI1 |
UniProt ID | J9Z6K5 |
| Length | 802 a.a. | PDB ID | |
| Note | TraI relaxase | ||
Relaxase protein sequence
Download Length: 802 a.a. Molecular weight: 87613.36 Da Isoelectric Point: 10.8339
>AFS51493.1 TraI relaxase (plasmid) [uncultured bacterium]
MIAKRIPMNSAKKSSFAGLVKYITSSQGINERVGQVRVTNCQTEELSWAVHEVLHTQKINTRAEGDKTYH
LLISLAPGEQPSSEVLRDIEDRVCSAIGYKDHQRVSAVHNDTDCLHIHIAINKIHPERHTLHEPYRDFKT
LGDICARLELEHGLQQVNHASKKSVSEGRANDMEQHAGIESLITWVRRECLEQVQGAKSWVELHQVMHEN
GLEMRERGNGLVITDQAGTMVKASTVARDLSKGKLEERLGAFENQQQQARASHKQGKPLPRKPGIGRVGR
RPPPASKNRLRGLSTLQTIQIDNGKRYEKSPLKHKSDTTELYARFRDEQNAVRHTKAKELAAARERRDRA
IEQAKNVAKLKRSSIKLMATPGLTKKLLYASTHKSLQEQITSLRAEHIKAIENINTLNKARQWADWLQAK
ALDGDQEALKALRARQGVKGLQGDAVAAKGQQHSAKAIPLQQDHITKEGTVIYRAGASAIRDDGSKLQLS
RGANFDGIETALRMAAARYGEKITITGSDQFKELVAQTAAIRSLPIKFDDPALEQRRQSLQQAIEKERSN
VGRTDRGRTAGPGAGVDGLHATRGGRGTGAGRTTGAGRGAGVRGSATGTQLSGRAGTATSGRITVRAPGG
TPKPGVARVGRKPPPESQNRLRALSKLGVVQLTQGSEVLLPRDVPGVVEHQGAKPDNGLRRDVSRSGGIA
AEGKTISSAQAARQYVAEREDKRAKGFDIPKHRIYNQADDGPATFAGLRQVEGQSMALLKRGDELLVLPV
NEATARRMKRMKLGDPVTANATGAIKSKGRSR
MIAKRIPMNSAKKSSFAGLVKYITSSQGINERVGQVRVTNCQTEELSWAVHEVLHTQKINTRAEGDKTYH
LLISLAPGEQPSSEVLRDIEDRVCSAIGYKDHQRVSAVHNDTDCLHIHIAINKIHPERHTLHEPYRDFKT
LGDICARLELEHGLQQVNHASKKSVSEGRANDMEQHAGIESLITWVRRECLEQVQGAKSWVELHQVMHEN
GLEMRERGNGLVITDQAGTMVKASTVARDLSKGKLEERLGAFENQQQQARASHKQGKPLPRKPGIGRVGR
RPPPASKNRLRGLSTLQTIQIDNGKRYEKSPLKHKSDTTELYARFRDEQNAVRHTKAKELAAARERRDRA
IEQAKNVAKLKRSSIKLMATPGLTKKLLYASTHKSLQEQITSLRAEHIKAIENINTLNKARQWADWLQAK
ALDGDQEALKALRARQGVKGLQGDAVAAKGQQHSAKAIPLQQDHITKEGTVIYRAGASAIRDDGSKLQLS
RGANFDGIETALRMAAARYGEKITITGSDQFKELVAQTAAIRSLPIKFDDPALEQRRQSLQQAIEKERSN
VGRTDRGRTAGPGAGVDGLHATRGGRGTGAGRTTGAGRGAGVRGSATGTQLSGRAGTATSGRITVRAPGG
TPKPGVARVGRKPPPESQNRLRALSKLGVVQLTQGSEVLLPRDVPGVVEHQGAKPDNGLRRDVSRSGGIA
AEGKTISSAQAARQYVAEREDKRAKGFDIPKHRIYNQADDGPATFAGLRQVEGQSMALLKRGDELLVLPV
NEATARRMKRMKLGDPVTANATGAIKSKGRSR
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | J9Z6K5 |
Reference
[1] Sen D et al. (2013) Inferring the evolutionary history of IncP-1 plasmids despite incongruence among backbone gene trees. Mol Biol Evol. 30(1):154-66. [PMID:22936717]
Auxiliary protein
| ID | 152 | GenBank | AFS51494 |
| Name | TraH_pMBUI1 |
UniProt ID | J9Z6C9 |
| Length | 139 a.a. | PDB ID | _ |
| Note | TraH DNA transfer protein | ||
Auxiliary protein sequence
Download Length: 139 a.a. Molecular weight: 15457.14 Da Isoelectric Point: 3.6758
>AFS51494.1 TraH DNA transfer protein (plasmid) [uncultured bacterium]
MSEELTEDELLAQALASMDSTPPGEDEEPGPGEPPEPGEVLEFGEAPPEPSFLDELEQPPVDESPFAPLE
EPQSLVLQELDESRRPKARTVCERCPNSVWFSSPKEVKCYCRVMFLVSWSTKEPNQITGCDGMFLGQEE
MSEELTEDELLAQALASMDSTPPGEDEEPGPGEPPEPGEVLEFGEAPPEPSFLDELEQPPVDESPFAPLE
EPQSLVLQELDESRRPKARTVCERCPNSVWFSSPKEVKCYCRVMFLVSWSTKEPNQITGCDGMFLGQEE
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | J9Z6C9 |
| ID | 153 | GenBank | AFS51495 |
| Name | TraJ_pMBUI1 |
UniProt ID | J9Z7C8 |
| Length | 140 a.a. | PDB ID | _ |
| Note | TraJ DNA transfer protein | ||
Auxiliary protein sequence
Download Length: 140 a.a. Molecular weight: 15396.93 Da Isoelectric Point: 9.5501
>AFS51495.1 TraJ DNA transfer protein (plasmid) [uncultured bacterium]
MLCEAGRLPPSEAAAKPQRESGMDEKKITRKDTHLRVPVLPEEKAKIEESARAAGKSVAAYMRAVGLGYE
LQSVIDSRLVLELAKVNADQGRLGGLLKMWLVSDKRLAGIEEEQLHRTIRVVLDRIGETQAALLELAKKA
MLCEAGRLPPSEAAAKPQRESGMDEKKITRKDTHLRVPVLPEEKAKIEESARAAGKSVAAYMRAVGLGYE
LQSVIDSRLVLELAKVNADQGRLGGLLKMWLVSDKRLAGIEEEQLHRTIRVVLDRIGETQAALLELAKKA
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | J9Z7C8 |
| ID | 154 | GenBank | AFS51496 |
| Name | TraK_pMBUI1 |
UniProt ID | J9Z8J6 |
| Length | 142 a.a. | PDB ID | _ |
| Note | TraK oriT-binding protein | ||
Auxiliary protein sequence
Download Length: 142 a.a. Molecular weight: 15579.99 Da Isoelectric Point: 10.7327
>AFS51496.1 TraK oriT-binding protein (plasmid) [uncultured bacterium]
MAKSGKSLSERIAERAKKKKQPGRAGKNRAAFLAVRDEVRQALDDGWPVKDVWETLYDEGAIAFRYDAFI
GYVKKLIRQPATVAPSIPVVQEAPARPVLPAKTPTAKPKPTPKPATRDPVVTKPSTPGSFSFDSTPRKED
LL
MAKSGKSLSERIAERAKKKKQPGRAGKNRAAFLAVRDEVRQALDDGWPVKDVWETLYDEGAIAFRYDAFI
GYVKKLIRQPATVAPSIPVVQEAPARPVLPAKTPTAKPKPTPKPATRDPVVTKPSTPGSFSFDSTPRKED
LL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | J9Z8J6 |
T4CP
| ID | 102 | GenBank | AFS51492 |
| Name | TraG_pMBUI1 |
UniProt ID | Q1H9X0 |
| Length | 638 a.a. | PDB ID | _ |
| Note | TraG coupling protein | ||
T4CP protein sequence
Download Length: 638 a.a. Molecular weight: 70160.41 Da Isoelectric Point: 8.9574
>AFS51492.1 TraG coupling protein (plasmid) [uncultured bacterium]
MISKDKFNNAVGPQVRAKKPGVGKVIPALSLLSIGVGLAAATQYFAYDFKYQDVLGPHVNHIYAPWSILT
WASKWYGTYPEAVMRAGSIGVLASGVGLLALAITKMIQANSSKANEYLHGSARWANKKDIQAAGLLQRGR
TALEVVTSKSPPTHTGVYVGAWLDENGVQHYLRHNGPEHILTYAPTRSGKGVGLVIPTLLSWPASTVVTD
LKGELWALTSGWRQKHANNKVLRFEPATSNGGVRWNPLDEIRIGTENEVGDVQNLATLIVDPDGKGLESH
WQKTSQALLVGLIMHVLYKSKIEGTLATLPEVDRILADPDRDLGELWMEMTTYGHVNGKNHPVVGSSARD
MMDRPEEEAGSVLSTAKSYLSLYRDPIVARNVSASEFKVKDLMNHDNPVSLYIVTQPNDKARLRPLVRVL
INMIIRLLADKMDFENGRPVAHYNHRLLAMLDEFPSLGKLEILQESLAFVAGYGIKCYLICQDINQLKSR
ETGYGPDETITSNCHVQNAYPPNRVETAEHLSKLTGQTTVVKEQITTSGRRTSALLGQVSRTMQEVQRPL
LTPDECLRMPGPVKSAAGDITQAGDMVVYMAGYPAIYGKQPLYFKDPVFQARASIPAPKASDKLRFVQDH
SQAEAITI
MISKDKFNNAVGPQVRAKKPGVGKVIPALSLLSIGVGLAAATQYFAYDFKYQDVLGPHVNHIYAPWSILT
WASKWYGTYPEAVMRAGSIGVLASGVGLLALAITKMIQANSSKANEYLHGSARWANKKDIQAAGLLQRGR
TALEVVTSKSPPTHTGVYVGAWLDENGVQHYLRHNGPEHILTYAPTRSGKGVGLVIPTLLSWPASTVVTD
LKGELWALTSGWRQKHANNKVLRFEPATSNGGVRWNPLDEIRIGTENEVGDVQNLATLIVDPDGKGLESH
WQKTSQALLVGLIMHVLYKSKIEGTLATLPEVDRILADPDRDLGELWMEMTTYGHVNGKNHPVVGSSARD
MMDRPEEEAGSVLSTAKSYLSLYRDPIVARNVSASEFKVKDLMNHDNPVSLYIVTQPNDKARLRPLVRVL
INMIIRLLADKMDFENGRPVAHYNHRLLAMLDEFPSLGKLEILQESLAFVAGYGIKCYLICQDINQLKSR
ETGYGPDETITSNCHVQNAYPPNRVETAEHLSKLTGQTTVVKEQITTSGRRTSALLGQVSRTMQEVQRPL
LTPDECLRMPGPVKSAAGDITQAGDMVVYMAGYPAIYGKQPLYFKDPVFQARASIPAPKASDKLRFVQDH
SQAEAITI
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q1H9X0 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2251..12825
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_0 | 1..1122 | - | 1122 | AFS51460 | TrfA1 replication initiation protein | - |
| Locus_1 | 1162..1521 | - | 360 | AFS51461 | single strand DNA-binding protein | - |
| Locus_2 | 1628..2038 | + | 411 | AFS51462 | TrbA transcriptional repressor | - |
| Locus_3 | 2251..3207 | + | 957 | AFS51463 | TrbB mating pair formation protein | virB11 |
| Locus_4 | 3241..3648 | + | 408 | AFS51464 | TrbC mating pair formation protein | virB2 |
| Locus_5 | 3651..3962 | + | 312 | AFS51465 | TrbD mating pair formation protein | virB3 |
| Locus_6 | 3959..6517 | + | 2559 | AFS51466 | TrbE mating pair formation protein | virb4 |
| Locus_7 | 6514..7233 | + | 720 | AFS51467 | TrbF mating pair formation protein | virB8 |
| Locus_8 | 7245..8135 | + | 891 | AFS51468 | TrbG mating pair formation protein | virB9 |
| Locus_9 | 8139..8606 | + | 468 | AFS51469 | TrbH mating pair formation protein | - |
| Locus_10 | 8613..10031 | + | 1419 | AFS51471 | TrbI mating pair formation protein | virB10 |
| Locus_11 | 10054..10839 | + | 786 | AFS51470 | TrbJ plasmid entry exclusion protein | virB5 |
| Locus_12 | 10851..11087 | + | 237 | AFS51472 | TrbK plasmid entry exclusion protein | - |
| Locus_13 | 11158..12825 | + | 1668 | AFS51473 | TrbL mating pair formation protein | virB6 |
| Locus_14 | 12838..13158 | + | 321 | AFS51474 | TrbM mating pair formation protein | - |
| Locus_15 | 13168..13797 | + | 630 | AFS51475 | TrbN mating pair formation protein | - |
| Locus_16 | 13797..14267 | + | 471 | AFS51476 | TrbO mating pair formation protein | - |
| Locus_17 | 14271..15008 | + | 738 | AFS51477 | TrbP mating pair formation protein | - |
| Locus_18 | 15018..15263 | + | 246 | AFS51478 | TrbS mating pair formation protein | - |
| Locus_19 | 15281..15943 | + | 663 | AFS51479 | hypothetical protein | - |
| Locus_20 | 15954..16280 | + | 327 | AFS51480 | hypothetical protein | - |
| Locus_21 | 16309..16572 | + | 264 | AFS51482 | hypothetical protein | - |
| Locus_22 | 16623..17069 | + | 447 | AFS51481 | hypothetical protein | - |
Host bacterium
| ID | 102 | GenBank | JQ432563 |
| Plasmid name | pMBUI1 | Incompatibility group | IncP1 |
| Plasmid size | 44304 bp | Coordinate of oriT [Strand] | 32348..32390 [+] |
| Host baterium | Uncultured bacterium |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |