Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100104
Name   oriT_pAsa1 in_silico
Organism   Aeromonas salmonicida subsp. salmonicida strain A449
Sequence Completeness      incomplete
NCBI accession of oriT (coordinates [strand])   AY301063 (3260..3278 [-], 19 nt)
oriT length   19 nt
IRs (inverted repeats)     _
Location of nic site      14..15
Conserved sequence flanking the
  nic site    		  CTGG|CTAA
Note   ColE2-type

  oriT sequence  


Download         Length: 19 nt

>oriT_pAsa1
TACGGGTGGGCTGGCTAAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Boyd J et al. (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]


Relaxase

ID   99 GenBank   AAP69887
Name   MobA_pAsa1 insolico UniProt ID   Q79IR8
Length   550 a.a. PDB ID   
Note   mobilization relaxase

  Relaxase protein sequence


Download         Length: 550 a.a.        Molecular weight: 59514.89 Da        Isoelectric Point: 7.0025

>AAP69887.1 mobilization relaxase (plasmid) [Aeromonas salmonicida subsp. salmonicida A449]
MLIKFLSHGTGSGARASAYLMGSHDHTGAPRAGVAVQRGDPVLFAAVADSLPFQHRYASAVISWATEEAP
TADEITAVLEDFEAVAFAGLEPEDVHLTAVQHDEPDGGVHVHILIPRVHLGTGKSFNPAPPGHRKAFDAV
RDKHNHEKGWARPDDPLRARLLRPDFEAFKDKSDKSAIKKQITEHLLGAAAQGVISNAAELQDYLQTSLG
CEITRTGADYTSIKPEGYPKAIRLTGELYGSSWTAERTLEREAQTAARAGIGRGGEVSESGARQAQERLR
DACGRRAEYNRGRYPGRDKGIAQTDHRLDRPIEGGLGAGPERGQHPQADLQRLAQSLAVRGMHLRGDRAG
ELGGIELDGQRGSAVEQRDQQRESHAGAASGRSELRPGGWQELHPRSDIGKNVRNAKWQDGGGDQVNDRI
RTPTDRAAGADGAAAPQRDDGLAAAALRATDVFGELAKAVGEHLGRVRDALRQLTHRIRGLGEEHKERAS
QHGPEFGPDQPGYRSSTWVIEQQCGRSQELAGELDQGAALIADRAAELRKEQRRDRGLSM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(61-247)


  Protein structure


Source ID Structure
AlphaFold DB Q79IR8

  Reference


[1] Boyd J et al. (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]


Auxiliary protein

ID   144 GenBank   AAP69888
Name   MobB_pAsa1 insolico UniProt ID   Q79IR7
Length   177 a.a. PDB ID   _
Note   mobilization protein

  Auxiliary protein sequence


Download         Length: 177 a.a.        Molecular weight: 19449.37 Da        Isoelectric Point: 6.9925

>AAP69888.1 mobilization protein (plasmid) [Aeromonas salmonicida subsp. salmonicida A449]
MDHLGQQKERLSERLKQRQEQELGEVEKLAKAVLDKLKKDLETRVDGELNTTVDAIRDGIKGLPKQITDS
TDPLKEVLEQARSEANTLKLISSVWLKALLFGVCISVGIGLGSWGVLSWMGNEVQRLNSEISSAKATLAQ
LPAGVSFVREDGKSYILAPTLGKTYETQSGKMAVEIK

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB Q79IR7

  Reference


[1] Boyd J et al. (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]

ID   145 GenBank   AAP69886
Name   MobC_pAsa1 insolico UniProt ID   Q7X2F0
Length   136 a.a. PDB ID   _
Note   mobilization protein

  Auxiliary protein sequence


Download         Length: 136 a.a.        Molecular weight: 14868.04 Da        Isoelectric Point: 11.1361

>AAP69886.1 mobilization protein (plasmid) [Aeromonas salmonicida subsp. salmonicida A449]
MSKVQKITTVSVRIDKPTAAEWRERASASGLSLSDWIRGAVDANQQTNLPTPQRRPVRVRDTSNDADPAL
MRQLAGMSNNLNQIARALNECRLAGSSVQLVEVLALLRSIEVQAGRLLPQLPPPPKPMAKGRDDAH

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(72-111)


  Protein structure


Source ID Structure
AlphaFold DB Q7X2F0

  Reference


[1] Boyd J et al. (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]

ID   146 GenBank   AAP69889
Name   MobD_pAsa1 insolico UniProt ID   Q7X2E9
Length   113 a.a. PDB ID   _
Note   mobilization protein

  Auxiliary protein sequence


Download         Length: 113 a.a.        Molecular weight: 12675.36 Da        Isoelectric Point: 6.4819

>AAP69889.1 mobilization protein (plasmid) [Aeromonas salmonicida subsp. salmonicida A449]
MTELERLLIERLEQMELRHHNETMDLRQQLYAQQMSLESLQRQLGSTLEGYETHCASLHTAFVALEKSTR
SELASMGRSLDQTNRVTGAVLGSLNSSVAGVKSSLESLIKAQR

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB Q7X2E9

  Reference


[1] Boyd J et al. (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]


Host bacterium

ID   99 GenBank   AY301063
Plasmid name   pAsa1 Incompatibility group   -
Plasmid size   5424 bp Coordinate of oriT [Strand]   3260..3278 [-]
Host baterium   Aeromonas salmonicida subsp. salmonicida strain A449

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -