Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100103
Name   oriT_pAsa3 in_silico
Organism   Aeromonas salmonicida subsp. salmonicida strain A449
Sequence Completeness      incomplete
NCBI accession of oriT (coordinates [strand])   AY301065 (3437..3455 [-], 19 nt)
oriT length   19 nt
IRs (inverted repeats)     _
Location of nic site      14..15
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   ColE2-type

  oriT sequence  


Download         Length: 19 nt

>oriT_pAsa3
TACGGGTAGGCTGGCTTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Boyd J et al. (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]


Relaxase

ID   98 GenBank   AAP69902
Name   MobA_pAsa3 insolico UniProt ID   Q7X2D9
Length   555 a.a. PDB ID   
Note   mobilization relaxase

  Relaxase protein sequence


Download         Length: 555 a.a.        Molecular weight: 60268.74 Da        Isoelectric Point: 6.5510

>AAP69902.1 mobilization relaxase (plasmid) [Aeromonas salmonicida subsp. salmonicida A449]
MHIKFLAHGTGSGAHASAYLMGPHDHTGEPRAGVAVLRGDPVLFAAVADSLPFQQRYSSAVISWAAEEAP
RPDEISAVLDDFEALAFAGLAREDVHLTAVEHAEADGGVHVHILIPRVHLGSGKSFNPAPPGQRKDFDAV
RDKHNHEKGWARPDDPLRARLLRPDFEAYKAVTDHTAIKKQLTDHLIGAVAQGVVSNADELRAYLQEGLG
CEITRTGKDYTSVKPEGYPKAIRLKGELYGESWTAQSTLEREAQAAARAGIGRGGEVSASAARDAQERLA
GAHQRRAEYNRDRYQGRDRGLAPADLRREQTLENSLEGGQGRSRHAETGLQRLVQSRAAGDFLRGGVTDG
KLVWHELDMGADRTRHGDVAEFAARDQQRPEHVGRVAEGGELLQGGGWKTLHHRPQDWGAVQDRGGQDGR
GGALNDRAGAGTVGEAGADRTAARATNRGLEAAIAAASALFERVAKGVQRCAQELREAMQRPARGDPDVA
ERGNALEQGYQRSARELEQQCQRLEQGAGELAERAGLINEQAKELKESKELELSLEYERYRGLEL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(61-248)


  Protein structure


Source ID Structure
AlphaFold DB Q7X2D9

  Reference


[1] Boyd J et al. (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]


Auxiliary protein

ID   141 GenBank   AAP69903
Name   MobB_pAsa3 insolico UniProt ID   Q7X2D8
Length   185 a.a. PDB ID   _
Note   mobilization protein

  Auxiliary protein sequence


Download         Length: 185 a.a.        Molecular weight: 20865.07 Da        Isoelectric Point: 5.1693

>AAP69903.1 mobilization protein (plasmid) [Aeromonas salmonicida subsp. salmonicida A449]
MESLGQRKALLSEKLKQRQEQELGEVEKLAQALLETLRSDLQERISGELNTTVTAIKEGIEDLPQQIYDA
SRPLKTVLREVKDEADMLKLVSSVWFKAALLGIFCGVGLLMGSWFGMNWIWEQIEQDMGTLRSLRQEISS
AQSTLDELPKGVSYFKGEDGKRYIIARKIGAPYKTEAGKMAVEVR

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB Q7X2D8

  Reference


[1] Boyd J et al. (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]

ID   142 GenBank   AAP69901
Name   MobC_pAsa3 insolico UniProt ID   Q7X2E0
Length   136 a.a. PDB ID   _
Note   mobilization protein

  Auxiliary protein sequence


Download         Length: 136 a.a.        Molecular weight: 15025.21 Da        Isoelectric Point: 10.4639

>AAP69901.1 mobilization protein (plasmid) [Aeromonas salmonicida subsp. salmonicida A449]
MTKTARTTTVSVRIDKPTAAEWREKAEAADLSLSDWIRGAIDANQQMHLPTPRKRAVRVRDTSHDADPVL
MRQLAGMSNNLNQIARALNECRLIGSPVQVVEVLALLRSIEGQATHLLPQLPPPPKPNRKGESDAH

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(72-111)


  Protein structure


Source ID Structure
AlphaFold DB Q7X2E0

  Reference


[1] Boyd J et al. (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]

ID   143 GenBank   AAP69904
Name   MobD_pAsa3 insolico UniProt ID   Q7X2D7
Length   102 a.a. PDB ID   _
Note   mobilization protein

  Auxiliary protein sequence


Download         Length: 102 a.a.        Molecular weight: 11221.62 Da        Isoelectric Point: 6.4712

>AAP69904.1 mobilization protein (plasmid) [Aeromonas salmonicida subsp. salmonicida A449]
MTELERVLLAKLEQIEQRHEQQTEDLRLQLQQQAHSLSALQKVCNDALRSCGKLCSDLHEEIRTLQSGVT
HSNKVTSAALGSLNSSVSALNKALENLQSAQG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB Q7X2D7

  Reference


[1] Boyd J et al. (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]


Host bacterium

ID   98 GenBank   AY301065
Plasmid name   pAsa3 Incompatibility group   -
Plasmid size   5616 bp Coordinate of oriT [Strand]   3437..3455 [-]
Host baterium   Aeromonas salmonicida subsp. salmonicida strain A449

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -