Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100102
Name   oriT_pAsa2 in_silico
Organism   Aeromonas salmonicida subsp. salmonicida strain A449
Sequence Completeness      incomplete
NCBI accession of oriT (coordinates [strand])   AY301064 (2790..2808 [-], 19 nt)
oriT length   19 nt
IRs (inverted repeats)     _
Location of nic site      14..15
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   ColE1 superfamily

  oriT sequence  


Download         Length: 19 nt

>oriT_pAsa2
TCTGTGTATGCTGGCTTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Boyd J et al. (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]


Relaxase

ID   97 GenBank   AAP69894
Name   MobA_pAsa2 insolico UniProt ID   Q7X2E5
Length   486 a.a. PDB ID   
Note   mobilization relaxase

  Relaxase protein sequence


Download         Length: 486 a.a.        Molecular weight: 53927.43 Da        Isoelectric Point: 6.3120

>AAP69894.1 mobilization relaxase (plasmid) [Aeromonas salmonicida subsp. salmonicida A449]
MIVKFHARGVGRGSGPVDYLLGPQRDREGATLDRGDPGLIEALIDSSPYAKKYTSGVLSFQEADLPREDK
DKLMDSFERALLPGLDRDQYACLWVEHRDKERLELNFVIPNIELLSGKRLQPYFDRADRPRIDAWKVVVN
AQLGLHDPDDPLNKRALMTPSNLPKAKQDAAQAITDGLLSLAAAGELADRGDVVATLKQHGFEVVRETRK
SISIADPEGGRNIRLKGALYEQDFRAGQDLRKELEAAGERYRNEREERVRAARESYSRGVGLKRAEIERR
YRRPEPEVGLAGVEGVGFSVDRGGISAGDQLGRGVVPGEGHRRELGGDLAPEQDIGAVDGEGRQIGTVNV
RRGQAAVREGGLEGVGLWRQRERRISLDDPGGLLNDRARTAVIERVREFGRRVQSAALGLGERLQRFAAD
VRGHLYREPGIAGERGELEQAGVALERSSREIEPKIEALSLDREPRCGGMSFGREYREYGDREYGD

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-242)


  Protein structure


Source ID Structure
AlphaFold DB Q7X2E5

  Reference


[1] Boyd J et al. (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]


Auxiliary protein

ID   138 GenBank   AAP69895
Name   MobB_pAsa2 insolico UniProt ID   Q79IR5
Length   154 a.a. PDB ID   _
Note   mobilization protein

  Auxiliary protein sequence


Download         Length: 154 a.a.        Molecular weight: 17517.17 Da        Isoelectric Point: 6.9597

>AAP69895.1 mobilization protein (plasmid) [Aeromonas salmonicida subsp. salmonicida A449]
MSKILELAKTFEKSSKQQASDIETSVKNAFEPHEKAILEALDSSGRRLNAAIDAQSRRWGWLVLKGWVFP
LIGVAFLLGISWVVVWYQGRVIAENWVEISRQNKTLEQLTAKGGKLELSTCGEDKRLCVKVDLKELAYGD
KEKDEYPWMIPEGY

  Protein domains


Predicted by InterproScan.

MbeB-like, N-term conserved region (MbeB_N)

(1-52)


  Protein structure


Source ID Structure
AlphaFold DB Q79IR5

  Reference


[1] Boyd J et al. (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]

ID   139 GenBank   AAP69893
Name   MobC_pAsa2 insolico UniProt ID   Q7X2E6
Length   105 a.a. PDB ID   _
Note   mobilization protein

  Auxiliary protein sequence


Download         Length: 105 a.a.        Molecular weight: 11490.40 Da        Isoelectric Point: 11.1592

>AAP69893.1 mobilization protein (plasmid) [Aeromonas salmonicida subsp. salmonicida A449]
MIKIRVTADELVRLKGLSGDERLAEWMRSKCLGNDKAAGRRRTPVPKADPALLRQIAGIGNNLNQVARRV
NSGEWGAVERVQVIAVLMAMERALQAISVPSTEVT

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(53-95)


  Protein structure


Source ID Structure
AlphaFold DB Q7X2E6

  Reference


[1] Boyd J et al. (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]

ID   140 GenBank   AAP69896
Name   MobD_pAsa2 insolico UniProt ID   Q79IR4
Length   74 a.a. PDB ID   _
Note   mobilization protein

  Auxiliary protein sequence


Download         Length: 74 a.a.        Molecular weight: 8418.51 Da        Isoelectric Point: 6.4635

>AAP69896.1 mobilization protein (plasmid) [Aeromonas salmonicida subsp. salmonicida A449]
MTELERQLLNALESLEGGYRVQHSAWESAYSDLRLMFEATSTENRALRESVGSLSKQVLHLSAQVERLSQ
KLKP

  Protein domains


Predicted by InterproScan.

MbeD/MobD like (MbeD_MobD)

(1-70)


  Protein structure


Source ID Structure
AlphaFold DB Q79IR4

  Reference


[1] Boyd J et al. (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]


Host bacterium

ID   97 GenBank   AY301064
Plasmid name   pAsa2 Incompatibility group   -
Plasmid size   5247 bp Coordinate of oriT [Strand]   2790..2808 [-]
Host baterium   Aeromonas salmonicida subsp. salmonicida strain A449

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -