Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100100
Name   oriT_pTF4.1 in_silico
Organism   Acidithiobacillus ferrooxidans
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NC_001520 (245..319 [-], 75 nt)
oriT length   75 nt
IRs (inverted repeats)      IR1:3..7, 19..23  (CCTTG..CAAGG)
  IR2: 49..58, 67..75  (CCACGGGGGA..TCCCCCTGG)
Location of nic site      29..30nt
Conserved sequence flanking the
  nic site    		  _
Note   _

  oriT sequence  


Download         Length: 75 nt

>oriT_pTF4.1
CTCCTTGCTCGGTTGGGTCAAGGGGCACCGCGCCCTTGCCTTGGGGGTCCACGGGGGAGCGGAGCATCCCCCTGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   95 GenBank   NP_052137
Name   ORF1_pTF4.1 insolico UniProt ID   Q56294
Length   542 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 542 a.a.        Molecular weight: 61320.55 Da        Isoelectric Point: 7.1304

>NP_052137.1 unnamed protein product (plasmid) [Acidithiobacillus ferrooxidans]
MEAFGAGTRCKSPLYHANIDPRADERLTPEQWGRAVDALEAKLGFTGQPRALVQHVKEGREHVHVVWSRI
DIERNRAIPDSHNYRKHEEVARALEREFGHERVQGAHVEREGKERPARTPSHAEMQQAERSGLDPKAIKA
EITALWRQTDSGKAFAGALAEAGYILARGDKRDFIVIDQAGESHSLARRIDGVKAADMRARLSDIDQATL
PDARGARAEQAQKALDDAQKAEEARTAEAREAAQKAAEAVERADEADLTRWRSMPLNALQWEINTLRPDR
YAALAAGQAAEPTYRETLEATDAAHAGAVKARGEALEATETVRSLKQKAERYREYQGLLGRVRFWLQDKG
ILEIPEWKRLQTEISKAQKLADKRQNDLATAEALHTEAKSALKTLDSAIAGAVDVAQAPEWRRYEVARSV
LNERIEEDRRRAAEAEAERRAEEIRRRVDAEIEREKREGIEQQRRAEISKIPTIVRGLASITQTEQGTFR
VRYPDLPSEELKRQQDLLRRYPDEAKSAVKAEFSERQVQAKREKDRGFDMMD

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(14-177)


  Protein structure


Source ID Structure
AlphaFold DB Q56294


Auxiliary protein

ID   136 GenBank   NP_052136
Name   ORF4_pTF4.1 insolico UniProt ID   Q56293
Length   121 a.a. PDB ID   _
Note   Bacterial mobilisation protein (MobC); pfam05713

  Auxiliary protein sequence


Download         Length: 121 a.a.        Molecular weight: 12524.27 Da        Isoelectric Point: 11.0590

>NP_052136.1 unnamed protein product (plasmid) [Acidithiobacillus ferrooxidans]
MASGSETRQRQAALRIRLTAAEHATITAASERAGLSLAGYARSQLLSAPPVRQARRPPVERAELARLLAE
LGKIGSNVNQIAHALNGGGDAVPSDLASVQADIAAIRAAIMLALGREPGEP

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(68-110)


  Protein structure


Source ID Structure
AlphaFold DB Q56293


Host bacterium

ID   95 GenBank   NC_001520
Plasmid name   pTF4.1 Incompatibility group   -
Plasmid size   4104 bp Coordinate of oriT [Strand]   245..319 [-]
Host baterium   Acidithiobacillus ferrooxidans

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -