Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100092 |
| Name | oriT_pSU233  |
| Organism | Escherichia coli |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | X55896 (105..383 [-], 279 nt) |
| oriT length | 279 nt |
| IRs (inverted repeats) | 220..227, 230..237 (GCAAAAAC..GTTTTTGC) |
| Location of nic site | 246..247 |
| Conserved sequence flanking the nic site |
GTGGGGTGT|GG |
| Note | _ |
oriT sequence
Download Length: 279 nt
>oriT_pSU233
CGCTAGCAGCGCCCCTGACGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGCTGTAGCTGCGTTAACGACGCTGCTTTAAATAAATCAGATTTAAACAATATAAATCCACAAATACAACTCNATGATATTAAAGATAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTT
CGCTAGCAGCGCCCCTGACGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGCTGTAGCTGCGTTAACGACGCTGCTTTAAATAAATCAGATTTAAACAATATAAATCCACAAATACAACTCNATGATATTAAAGATAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTT
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Salazar L et al. (1992) Characterization and nucleotide sequence of the oriT-traM-finP region of the IncFVII plasmid pSU233. Mol Gen Genet . 234(3):442-8. [PMID:1406590]
Auxiliary protein
| ID | 126 | GenBank | CAA39383 |
| Name | TraM_pSU233  |
UniProt ID | P33787 |
| Length | 127 a.a. | PDB ID | _ |
| Note | _ | ||
Auxiliary protein sequence
Download Length: 127 a.a. Molecular weight: 14525.54 Da Isoelectric Point: 4.8662
>CAA39383.1 traM protein (plasmid) [Escherichia coli]
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVYEAQMERKESAFNQAEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSVEMERFFPKNDDE
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVYEAQMERKESAFNQAEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSVEMERFFPKNDDE
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | P33787 |
Reference
[1] Salazar L et al. (1992) Characterization and nucleotide sequence of the oriT-traM-finP region of the IncFVII plasmid pSU233. Mol Gen Genet . 234(3):442-8. [PMID:1406590]
Host bacterium
| ID | 87 | GenBank | X55896 |
| Plasmid name | pSU233 | Incompatibility group | IncFVII |
| Plasmid size | 1264 bp | Coordinate of oriT [Strand] | 105..383 [-] |
| Host baterium | Escherichia coli |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |