Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100088
Name   oriT_pRJ6 experimental
Organism   Staphylococcus aureus
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_011522 (2061..2299 [-], 239 nt)
oriT length   239 nt
IRs (inverted repeats)      IR1: 1..8, 14..21  (AGTGGCTA..TAGCCACT)
  IR2: 22..29, 34..41  (ATTTTTTC..GAAAAAAT)
  IR3: 94..98, 104..108  (TGGCT..AGCCA)
Location of nic site      115..116
Conserved sequence flanking the
  nic site    		  TGCTTG|CCA
Note   The oriT includes three MobC binding sites, a MobA binding site and a nick site. Small Staphylococcal Plasmid, ColE1 superfamily

  oriT sequence  


Download         Length: 239 nt

>oriT_pRJ6
AGTGGCTAGCAATTAGCCACTATTTTTTCGTCAGAAAAAATCCTAAGGGGCTTGGGGATATTCCCCAACAAGCAGGCGCGTCTGCCACACTAGTGGCTAGCAAAGCCAATGCTTGCCAAACCACTATAACGACTTGATTAAATTTACTTTATCCTATAAAGTAAATTTATATTAAGGAGTTATCTTTCGTAGAAAGCGTGGTGGGATGTATGAGCGAACAAAACATATTTGTGGCTAGC

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Varella Coelho ML et al. (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]


Relaxase

ID   83 GenBank   YP_002302331
Name   MobA_pRJ6 insolico UniProt ID   B6GUP5
Length   325 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 325 a.a.        Molecular weight: 37588.51 Da        Isoelectric Point: 8.9045

>YP_002302331.1 MobA (plasmid) [Staphylococcus aureus]
MATTKLSATKSTSRAINYAEKRAVVKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVIIQAFKPDEVTPE
QCNQLGLELAEKFAPNHQVAVYTHNDTDHVHNHIVINSINLETGKKFNNNKQALRDLRDFNDELCRNHGL
SVPEKDTARLRYTQTEKAIANPNTKSTAQYSWKDEIREAIDQSQATNIDELKTHLRQYGIEIERVTLKSI
TYRHLAEDKKVRGRRLGEDYNKGGIEDGFERQIQRRQQKRRTSNRDEATERDTGITQSDWDRFAKDTNEL
ERSRKAAESARFINEKARRDREERIQTERTTRTIINNDRDDGYEL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-242)


  Protein structure


Source ID Structure
AlphaFold DB B6GUP5

  Reference


[1] Varella Coelho ML et al. (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]


Auxiliary protein

ID   121 GenBank   YP_002302330
Name   MobB_pRJ6 insolico UniProt ID   B6GUP4
Length   261 a.a. PDB ID   _
Note   mobilization associated protein

  Auxiliary protein sequence


Download         Length: 261 a.a.        Molecular weight: 30869.60 Da        Isoelectric Point: 8.6517

>YP_002302330.1 MobB (plasmid) [Staphylococcus aureus]
MALKDKYNDDNKNDELRIVMKQLKEIQVSLNQIGIDSPRIQMNWSVAEKLQNQQDLLMKRLEEIEKREFK
RNERHEQLLTTIETTATNFDQATKTNEKRFISAMKHYIQRIDSDNLKQDFTTAMQEELKDIKSETENMIE
QFQASQVELQRSNNEYKKTIEARLNKNETAIKQYDQAVNKLTKGLTSMFFIVTLVMVACLVLSPLSDVLG
INYFYEWINHVLKTGHSAWRYFIVILYLIPYILLWLLIRGILNAYKRVSSF

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB B6GUP4

  Reference


[1] Varella Coelho ML et al. (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]

ID   122 GenBank   YP_002302333
Name   MobC_pRJ6 insolico UniProt ID   Q93GG1
Length   127 a.a. PDB ID   _
Note   mobilization associated protein

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14552.54 Da        Isoelectric Point: 10.2484

>YP_002302333.1 MobC (plasmid) [Staphylococcus aureus]
MSEQNIFVASDTVNGQIHKPHRKAPKQISFRVSESEYLKLKQSAETLNMSVPAFVKKKAQGTRLVAPKLD
KVTRQSIARDLGKLGSNANQIAKYCNQHQYDAPNYEALERNISELRERLDDIWQRLN

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(79-121)


  Protein structure


Source ID Structure
AlphaFold DB Q93GG1

  Reference


[1] Varella Coelho ML et al. (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]


T4CP

ID   138 GenBank   AAZ50583
Name   TraG_pTiBo542 insolico UniProt ID   A5WYB5
Length   647 a.a. PDB ID   _
Note   conjugal transfer coupling protein TraG

  T4CP protein sequence


Download         Length: 647 a.a.        Molecular weight: 69615.82 Da        Isoelectric Point: 9.3647

>AAZ50583.1 TraG (plasmid) [Agrobacterium tumefaciens]
MRANRLLLAILPAAIMLAAMILTSGMEYRLSALGTSAQAKLMLGRAGLALPHVVAAAIGVVALFAANGST
HIKVAGLSVLAGSAVVIIIAMTRETIRLAGVAGNVPAGQTVLAYADPATMIGVGVAFIGSVFALRVAIKG
NAAFAMAAPKRIGGRRAVHGEADWMKMQEAGTLFPEHGGIVIGERYRVDRDSVAAMPFRADDKQSWGGGG
KAPLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGGLVVLDPSSEVAPMVSEHRRQAGRKVIVLD
PTASGVGFNALDWIGRHGNTKEEDIVAVATWIMTDNARTASARDDFFRASAMQLLTALIADVCLSGHTDE
KEQTLRRVRANLSEPEPKLRARLTKIYEQSESDFVKENVAVFVNMTPETFSGVYANAVKETHWLSYTNYA
ALVSGDSLSTDDLAEGGTDIFIALDLKVLEAHPGLARVVIGSLLNAIYNRNGDVKGRTLFLLDEVARLEF
LRILETARDAGRKYGITLTLIFQSLGQMREAYGGRDATSKWFESASWISFSAINDPDTADYISKRCGDTT
VEVDQTNRSSGMKGSSRSRSKQLNRRPLILPHEVLRMRADEQIVFTSGNAPLRCGRAMWFRREDMKACVA
ENRFQKNSFGTDNPALN

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(163-638)

  Protein structure


Source ID Structure
AlphaFold DB A5WYB5


Host bacterium

ID   83 GenBank   NC_011522
Plasmid name   pRJ6 Incompatibility group   _
Plasmid size   7904 bp Coordinate of oriT [Strand]   2061..2299 [-]
Host baterium   Staphylococcus aureus

Cargo genes

Drug resistance gene   resistance to aureocin A70
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -