Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100086
• Name: oriT_pIP1630
• Organism: Staphylococcus epidermidis
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): AF045472 (397..511 [+], 115 nt)
• oriT length: 115 nt
• IRs (inverted repeats): IR1: 1..7, 11..17 (TTGGGGATATTCCCCAA) IR2: 30..37, 42..50 (GCTACCAC..GTGGCTAGC) IR3: 89..100, 104..115 (ATTTTACTTTAT..ATAAAGTAAAAT)
• Location of nic site: 64..65
• Conserved sequence flanking the nic site: TGCTTG|CCA
• Note: Small Staphylococcal Plasmid, ColE1 superfamily

oriT sequence

• Length: 115 nt

>oriT_pIP1630
TTGGGGATATTCCCCAACAAGCAGGCGTCGCTACCACGTGAGTGGCTAGCAAAGCCAGTGCTTGCCAAACCACTTAACGACTTGATGAATTTTACTTTATCCTATAAAGTAAAAT

oriT secondary structure

Predicted by RNAfold.

Reference

[1] Varella Coelho ML et al. (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]

Relaxase

MobA2_pIP1630

• ID: 81
• GenBank: AAD02405
• Name: MobA2_pIP1630
• UniProt ID: Q9R968
• Length: 337 a.a.
• Note: relaxase

Relaxase protein sequence

• Length: 337 a.a.
• Molecular weight: 38606.52 Da
• Isoelectric Point: 7.3199

>AAD02405.1 mobilization protein (plasmid) [Staphylococcus epidermidis]
MMATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVIIQSFKPGEVTP
EQCNQLGLALAEKLVPNHQVAVYTHNDTDHVHNHIVINSIDLETGKKFNNNKQALRNVRNFNDEVCMEHG
LSVPEKNTARLRYTQTEKAIADPNTKSTAQYSWKDEIREAIDQSQATNMDEFKDHLNQHGIEIERVTPKT
ITYRHLAEDKKVRGRKLGEDYNKGGIEDGFERQIQHQQQTERVSDTDVQPKRRTSSRDESTERDTGVTQA
DWDQFAQDTNELERSRQAAESARLADEKARRDREKRERAKQTSRRIINNDRDHGLEL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (10-243)

Protein structure

• AlphaFold DB: Q9R968

Reference

[1] Varella Coelho ML et al. (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]

Auxiliary protein

MobB2_pIP1630

• ID: 117
• GenBank: AAD02406
• Name: MobB2_pIP1630
• UniProt ID: Q9ZI04
• Length: 269 a.a.
• PDB ID: _
• Note: mobilization protein

Auxiliary protein sequence

• Length: 269 a.a.
• Molecular weight: 31625.15 Da
• Isoelectric Point: 8.2764

>AAD02406.1 mobilization protein (plasmid) [Staphylococcus epidermidis]
MALKDKYNTNNKQNEYQTPTSNPNDELQVVMNQLNEIQVSLKQIGTNSPKTQMSLSVADKLQSQQDLLMK
RLEEIEKRENERNKRHDELLTTIETTASNFNQATEITQKRFISVAKHYIERINNDNLKQDFQTAIQEELK
DVKTDTHKAIEQLQTNQSELRQANNDYKKMIDERIKHNDTAMKQYDQAFNRLTKGITAMFFIIALVMVAF
LVLSPLGDWLGVQHFYEWLNYVLKTGHSVWRYLIVIFYLVPYVFFGMLIYAILSAYKRI

Protein domains

No domain identified.

Protein structure

• AlphaFold DB: Q9ZI04

Reference

[1] Varella Coelho ML et al. (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]

MobC2_pIP1630

• ID: 118
• GenBank: AAD02403
• Name: MobC2_pIP1630
• UniProt ID: P95736
• Length: 127 a.a.
• PDB ID: _
• Note: Bacterial mobilisation protein (MobC); pfam05713

Auxiliary protein sequence

• Length: 127 a.a.
• Molecular weight: 14471.40 Da
• Isoelectric Point: 10.1833

>AAD02403.1 mobilization protein (plasmid) [Staphylococcus epidermidis]
MSEQNTFVASDETVGRNRKPNRKAPKQISFRVSESEYLKLQQSAETLNMSVPAFVKKKAQGARLVAPKLD
QATRQSVAKDLSMLGANANQIAKYCNQHQHEAPNYEALERNISELRERLDEIWKKLN

Protein domains

Predicted by InterproScan.

• Bacterial mobilisation protein (MobC) (MobC) (81-122)

Protein structure

• AlphaFold DB: P95736

Reference

[1] Varella Coelho ML et al. (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]

Host bacterium

• ID: 81
• GenBank: AF045472
• Plasmid name: pIP1630
• Incompatibility group: -
• Plasmid size: 7263 bp
• Coordinate of oriT [Strand]: 397..511 [+]
• Host baterium: Staphylococcus epidermidis

Cargo genes

• Drug resistance gene: resistance to trimethoprim
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -