Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100085
Name   oriT_pSJ7.4 experimental
Organism   Neisseria gonorrhoeae
Sequence Completeness      incomplete
NCBI accession of oriT (coordinates [strand])   AY221996 (1186..1240 [+], 55 nt)
oriT length   55 nt
IRs (inverted repeats)      IR1: 16..24, 28..36  (CAGTTTCTC..GAGAAACTG)
  IR2: 43..47, 51..55  (AAGCG..CGCTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   _

  oriT sequence  


Download         Length: 55 nt

>oriT_pSJ7.4
GTAATGCCCACTTATCAGTTTCTCTTCGAGAAACTGGTGGGCAAGCGTACCGCTT

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Rodriguez-Bonano NM et al. (2004) Molecular analysis of oriT and MobA protein in the 7.4 kb mobilizable beta-lactamase plasmid pSJ7.4 from Neisseria gonorrhoeae. Plasmid. 52(2):89-101. [PMID:15336487]


Relaxase

ID   80 GenBank   AAO45530
Name   MobA_pSJ7.4 insolico UniProt ID   Q84AT6
Length   478 a.a. PDB ID   
Note   MobA; relaxase

  Relaxase protein sequence


Download         Length: 478 a.a.        Molecular weight: 56206.84 Da        Isoelectric Point: 8.1947

>AAO45530.1 mobilization protein A (plasmid) [Neisseria gonorrhoeae]
MAIYHLNVRYCSKSKGQSAQAKNDYINRNDKYSKRLDDLQFSGYGNMPKFAEDNPQEFWRLSDIYERANA
RVCTEIEFALPRELTLEQQQKLVSSFIENTVDSGSNKLPYSFAIHTDKNNHNPHCHLIFSERQLDGIDRT
AEQFFKRANTKSPEKGGAMKTADFRDREFIQSVRKTWREQANQALEQYGYAARIDERSYKEQGIEQAPRA
RIDRVTWQELNRLEQEERQIVQELALKGQEINKEKSYLQKIEEKQAQGMGKYESKFAAAFSKLSESALKH
DLSNEKEKDSKIHTQEEKVPQNRIQGLSQADFDQFLIDEWLPQIEKYVKAQEKRDGMEVEITQYDKDLQR
IQGDYNKLTDKNQGFLGLWETKEQKAKKKELEDEYKHTAEQRNAKSQELAEYSQKIKAYEQKTLEPINEK
IAKYQADNPEIKMRSLGFVKKIKAQGAYKAAQERMEREKQHQQEKQQRHLERESGLSL

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(23-211)


  Protein structure


Source ID Structure
AlphaFold DB Q84AT6

  Reference


[1] Rodriguez-Bonano NM et al. (2004) Molecular analysis of oriT and MobA protein in the 7.4 kb mobilizable beta-lactamase plasmid pSJ7.4 from Neisseria gonorrhoeae. Plasmid. 52(2):89-101. [PMID:15336487]


Auxiliary protein

ID   116 GenBank   AAO45531
Name   MobC_pSJ7.4 insolico UniProt ID   Q84AT5
Length   98 a.a. PDB ID   _
Note   mobilization protein C

  Auxiliary protein sequence


Download         Length: 98 a.a.        Molecular weight: 11613.43 Da        Isoelectric Point: 10.3336

>AAO45531.1 mobilization protein C (plasmid) [Neisseria gonorrhoeae]
MASEKLTRLEQQQADLQRKQDEIKAKIRAIKSKEQAEKRKKATHYKVLLGAFWLNEIINNPLLDVDRNFP
KLRKFINDDKKFDELSAFINEEIEKGKK

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB Q84AT5

  Reference


[1] Rodriguez-Bonano NM et al. (2004) Molecular analysis of oriT and MobA protein in the 7.4 kb mobilizable beta-lactamase plasmid pSJ7.4 from Neisseria gonorrhoeae. Plasmid. 52(2):89-101. [PMID:15336487]


Host bacterium

ID   80 GenBank   AY221996
Plasmid name   pSJ7.4 Incompatibility group   -
Plasmid size   3212 bp Coordinate of oriT [Strand]   1186..1240 [+]
Host baterium   Neisseria gonorrhoeae

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -