Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100084 |
| Name | oriT_pNM5  |
| Organism | Uncultured marine bacterium |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | JX863070 (3401..3435 [+], 35 nt) |
| oriT length | 35 nt |
| IRs (inverted repeats) | 1..6, 10..15 (CCAGAA..TTCTGG) |
| Location of nic site | 24..25 |
| Conserved sequence flanking the nic site |
TACCGTG|TTAT |
| Note | _ |
oriT sequence
Download Length: 35 nt
>oriT_pNM5
CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Alippi AM et al. (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]
Relaxase
| ID | 79 | GenBank | AFU63384 |
| Name | Mob_pNM5 |
UniProt ID | K4IDV6 |
| Length | 444 a.a. | PDB ID | |
| Note | nicks plasmid at oriT to initiate conjugation | ||
Relaxase protein sequence
Download Length: 444 a.a. Molecular weight: 51819.84 Da Isoelectric Point: 6.6297
>AFU63384.1 Mob (plasmid) [uncultured marine bacterium]
MLFLTENEVSEIAHVQKYTKANMQGLSIHLDRKTENHSNEDIDIERSHLNYDLCEKEGDTLSRMNERLDE
VYCMDRKDVKACCSWVVTLPENLKDAPDEHQRKFFEETYGFLADRYGGEKNVLSANVHNDETTPHMHFAF
IPVVYDPKKEREKVSAKLVINRNELKVFHKDLDVHLKESIPEIYQDGILNDKTIGLDDIESIKKHSKEIE
AKKADMSKELEEFSRKEKEEMVKDLATFKKPDEIIKRIDSTSKESLLGGKTNLITKDYKQLIKVAKSAIK
EKQNLYNYKKVSSEKIGDLEATVRFADRKIKNLEGKNENLEKEVAGLQEHRINEIVFKSMLQDDNRDLNI
SQTEIDGRLILFNLENGHEPLDREQGERWSSTLEENKRLKTIPQSRLQRGLDKLKQFIEKFLVRGFSMDS
VLQKVEQEKSRKPKNKKKSHDLEL
MLFLTENEVSEIAHVQKYTKANMQGLSIHLDRKTENHSNEDIDIERSHLNYDLCEKEGDTLSRMNERLDE
VYCMDRKDVKACCSWVVTLPENLKDAPDEHQRKFFEETYGFLADRYGGEKNVLSANVHNDETTPHMHFAF
IPVVYDPKKEREKVSAKLVINRNELKVFHKDLDVHLKESIPEIYQDGILNDKTIGLDDIESIKKHSKEIE
AKKADMSKELEEFSRKEKEEMVKDLATFKKPDEIIKRIDSTSKESLLGGKTNLITKDYKQLIKVAKSAIK
EKQNLYNYKKVSSEKIGDLEATVRFADRKIKNLEGKNENLEKEVAGLQEHRINEIVFKSMLQDDNRDLNI
SQTEIDGRLILFNLENGHEPLDREQGERWSSTLEENKRLKTIPQSRLQRGLDKLKQFIEKFLVRGFSMDS
VLQKVEQEKSRKPKNKKKSHDLEL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | K4IDV6 |
Reference
[1] Alippi AM et al. (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]
Host bacterium
| ID | 79 | GenBank | JX863070 |
| Plasmid name | pNM5 | Incompatibility group | - |
| Plasmid size | 5109 bp | Coordinate of oriT [Strand] | 3401..3435 [+] |
| Host baterium | Uncultured marine bacterium |
Cargo genes
| Drug resistance gene | resistance to tetracycline |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |