Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100080 |
| Name | oriT_pBM02  |
| Organism | Lactococcus lactis subsp. cremoris strain P8-2-47 |
| Sequence Completeness | incomplete |
| NCBI accession of oriT (coordinates [strand]) | AY026767 (248..277 [+], 30 nt) |
| oriT length | 30 nt |
| IRs (inverted repeats) | 1..12, 19..30 (AAGTAAAGTATA..TATACTTTACTT) |
| Location of nic site | 17..18 |
| Conserved sequence flanking the nic site |
TAGTGTG|CTAT |
| Note | homologous to that of plasmid pMV158 |
oriT sequence
Download Length: 30 nt
>oriT_pBM02
AAGTAAAGTATAGTGTGCTATACTTTACTT
AAGTAAAGTATAGTGTGCTATACTTTACTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Sánchez C et al. (2003) Sequence and analysis of pBM02, a novel RCR cryptic plasmid from Lactococcus lactis subsp cremoris P8-2-47. Plasmid. 49(2):118-29. [PMID:12726765]
Relaxase
| ID | 75 | GenBank | AAK13009 |
| Name | Mob_pBM02 |
UniProt ID | Q939P5 |
| Length | 304 a.a. | PDB ID | |
| Note | relaxase | ||
Relaxase protein sequence
Download Length: 304 a.a. Molecular weight: 35379.14 Da Isoelectric Point: 6.6266
>AAK13009.1 Mob-like protein (plasmid) [Lactococcus cremoris]
MSMMVATMKKMKNGNLGGIQKHNQREFENHSNEDIDPERSDMNYDLVNDEKINYNEKVNQIIDEQRVGTR
AIRKDAVKVDEWIISSDREFFNNLDSEQTKEFFQSVVDYFSENFGKQNVAYANVHLDETTPHMHMGVVPM
KDGKLSSKTVFTREKLTEIQEQLPQYLNAKGFDIERGIEGSKSKHHDTSEYKKLKENITKEQFEDLSYKL
DQSEKRNIVYQDILENDFKVKTIEPREMKARLVLNDLERGIQPSNLDQAKEWLRNLKNAVGTKIDPSRLV
KGIDKLEKIIKVVFKVVKGMTLGL
MSMMVATMKKMKNGNLGGIQKHNQREFENHSNEDIDPERSDMNYDLVNDEKINYNEKVNQIIDEQRVGTR
AIRKDAVKVDEWIISSDREFFNNLDSEQTKEFFQSVVDYFSENFGKQNVAYANVHLDETTPHMHMGVVPM
KDGKLSSKTVFTREKLTEIQEQLPQYLNAKGFDIERGIEGSKSKHHDTSEYKKLKENITKEQFEDLSYKL
DQSEKRNIVYQDILENDFKVKTIEPREMKARLVLNDLERGIQPSNLDQAKEWLRNLKNAVGTKIDPSRLV
KGIDKLEKIIKVVFKVVKGMTLGL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q939P5 |
Reference
[1] Sánchez C et al. (2003) Sequence and analysis of pBM02, a novel RCR cryptic plasmid from Lactococcus lactis subsp cremoris P8-2-47. Plasmid. 49(2):118-29. [PMID:12726765]
Host bacterium
| ID | 75 | GenBank | AY026767 |
| Plasmid name | pBM02 | Incompatibility group | - |
| Plasmid size | 3854 bp | Coordinate of oriT [Strand] | 248..277 [+] |
| Host baterium | Lactococcus lactis subsp. cremoris strain P8-2-47 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |