Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100073 |
| Name | oriT_pMA67  |
| Organism | Paenibacillus larvae |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NC_010875 (3425..3459 [+], 35 nt) |
| oriT length | 35 nt |
| IRs (inverted repeats) | 1..6, 10..15 (CCAGAA..TTCTGG) |
| Location of nic site | 24..25 |
| Conserved sequence flanking the nic site |
TACCGTG|TTAT |
| Note | _ |
oriT sequence
Download Length: 35 nt
>oriT_pMA67
CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] You Y et al. (2012) Detection of a common and persistent tet(L)-carrying plasmid in chicken-waste-impacted farm soil. Appl Environ Microbiol. 78(9):3203-13. [PMID:22389375]
Relaxase
| ID | 69 | GenBank | YP_001966010 |
| Name | Mob_pMA67 |
UniProt ID | A0SGK6 |
| Length | 435 a.a. | PDB ID | |
| Note | nicks plasmid at oriT to initiate conjugation | ||
Relaxase protein sequence
Download Length: 435 a.a. Molecular weight: 51963.86 Da Isoelectric Point: 6.6291
>YP_001966010.1 Mob (plasmid) [Paenibacillus larvae]
MAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDERLSEVYCMNRKDVKV
CCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSANVHNDETTPHMHFDFVPVVWDEKKER
EKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQEGILNDKTIGIENIHSLKKYSAEIEQQKEEMANEFK
SFKYPQKVLKSIEREVEKKTVGVFNKKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEIKNLKV
NVKNFEEDNEHLRYRNIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLHNLEND
RMPKNKEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKELSQQRH
DRRKRKADLVAWSCN
MAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDERLSEVYCMNRKDVKV
CCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSANVHNDETTPHMHFDFVPVVWDEKKER
EKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQEGILNDKTIGIENIHSLKKYSAEIEQQKEEMANEFK
SFKYPQKVLKSIEREVEKKTVGVFNKKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEIKNLKV
NVKNFEEDNEHLRYRNIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLHNLEND
RMPKNKEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKELSQQRH
DRRKRKADLVAWSCN
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0SGK6 |
Reference
[1] You Y et al. (2012) Detection of a common and persistent tet(L)-carrying plasmid in chicken-waste-impacted farm soil. Appl Environ Microbiol. 78(9):3203-13. [PMID:22389375]
Host bacterium
| ID | 69 | GenBank | NC_010875 |
| Plasmid name | pMA67 | Incompatibility group | _ |
| Plasmid size | 5030 bp | Coordinate of oriT [Strand] | 3425..3459 [+] |
| Host baterium | Paenibacillus larvae |
Cargo genes
| Drug resistance gene | resistance to tetracycline |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |