Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 100072 |
Name | oriT_pSU1 |
Organism | Sporosarcina ureae |
Sequence Completeness | core |
NCBI accession of oriT (coordinates [strand]) | NC_014015 (3425..3459 [+], 35 nt) |
oriT length | 35 nt |
IRs (inverted repeats) | 1..6, 10..15 (CCAGAA..TTCTGG) |
Location of nic site | 24..25 |
Conserved sequence flanking the nic site |
TACCGTG|TTAT |
Note | _ |
oriT sequence
Download Length: 35 nt
>oriT_pSU1
CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileReference
[1] You Y et al. (2012) Detection of a common and persistent tet(L)-carrying plasmid in chicken-waste-impacted farm soil. Appl Environ Microbiol. 78(9):3203-13. [PMID:22389375]
Relaxase
ID | 68 | GenBank | YP_003560377 |
Name | Mob_pSU1 | UniProt ID | D5KLZ0 |
Length | 439 a.a. | PDB ID | |
Note | nicks plasmid at oriT to initiate conjugation |
Relaxase protein sequence
Download Length: 439 a.a. Molecular weight: 52396.34 Da Isoelectric Point: 6.3441
>YP_003560377.1 Mob (plasmid) [Sporosarcina ureae]
MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDERLSEVYCMNR
KDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSANVHNDETTPHMHFDFVPVVWD
EKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQEGILNDKTIGIENIHSLKKYSAEIEQQKEEM
ANEFKSFKYPQKVLKSIEREVEKKTVGVFNKKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEI
KNLKVNVKNFEEDNEHLRYRNIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLH
NLENDRMPKNKEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL
SQQRQQQKKTKSRSSGMEL
MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDERLSEVYCMNR
KDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSANVHNDETTPHMHFDFVPVVWD
EKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQEGILNDKTIGIENIHSLKKYSAEIEQQKEEM
ANEFKSFKYPQKVLKSIEREVEKKTVGVFNKKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEI
KNLKVNVKNFEEDNEHLRYRNIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLH
NLENDRMPKNKEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL
SQQRQQQKKTKSRSSGMEL
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | D5KLZ0 |
Reference
[1] You Y et al. (2012) Detection of a common and persistent tet(L)-carrying plasmid in chicken-waste-impacted farm soil. Appl Environ Microbiol. 78(9):3203-13. [PMID:22389375]
Host bacterium
ID | 68 | GenBank | NC_014015 |
Plasmid name | pSU1 | Incompatibility group | _ |
Plasmid size | 5031 bp | Coordinate of oriT [Strand] | 3425..3459 [+] |
Host baterium | Sporosarcina ureae |
Cargo genes
Drug resistance gene | resistance to tetracycline |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |