Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100067
Name   oriT_pPsv48B experimental
Organism   Pseudomonas savastanoi pv. savastanoi
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_019266 (37837..38057 [+], 221 nt)
oriT length   221 nt
IRs (inverted repeats)      IR1: 78..91, 102..117  (GATGTATACGGGGG..CCCCCCGAATACATCG)
  IR2: 124..131, 135..142  (CAAAGGGG..CCCCTTTG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   

  oriT sequence  


Download         Length: 221 nt

>oriT_pPsv48B
CTGTGTCATCGAGAGACATTCAGAGACAATCTTGGGTCTGACCCAAAATCTCGAATTTGCTAAACAGGCACGTTACCGATGTATACGGGGGGATGAAGCGCCCCCCCGAATACATCGCCGTGCCAAAGGGGAAACCCCTTTGAAACCCCGCAGAGCGGTAGCGCTTCGCTTGTCGTTTGTCATCAGTCGATAAATCAATTGCGTCATTGATCGTCGGAGGC

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Maite Añorga et al. (2022) Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335. Frontiers in microbiology. 13:1076710. [PMID:36578579]


Relaxase

ID   1082 GenBank   CBZ40029
Name   MOBPpPsv48B experimental UniProt ID   H1ZX26
Length   1257 a.a. PDB ID   
Note   

  Relaxase protein sequence


Download         Length: 1257 a.a.        Molecular weight: 138967.69 Da        Isoelectric Point: 9.5049

>CBZ40029.1 putative relaxase, mobilization protein (plasmid) [Pseudomonas savastanoi pv. savastanoi]
MLIRPGGYNTGAQEYLEEGNKSGREFTRDELDHRLVISGDLDLTRSIYESIPDRGQDRYLTFTLSFREDA
VAESTLKAVTAEFKQFLMYAYKAEEFNFYAEAHLPKIKYVTDKKTGKPVERKPHIHVIVPRINLLSGNEA
NPVGFYKNHEKYFEAFQEYLNQKYNLASPREHVRVDIADAASVLSRYKGDDFYGKNREFKQTLVKQVIEK
NVTTREAFYELAATYGETRIRNQGKDNEYVAVKLPGDAKFTNLKETIFHDDFIVRRDLKKEPLDKAIIAQ
RLTEWPQRAMEIKYVEKATPAFRKRYVAASPEERQQLLAEREQKFYQVHGEHNDNVHTGQRQRDHQRSPS
ETAGRRTAAPADGLQDLSVSDVADHRQAGSARSRDGALLLPSDAHVHLGQSQPGGDSGLRPSVPGGGRGR
GSRSTTERGRGGSGPAAVSQEATGTATPTGATGRRRAGAGKPRNSRVRAGRVVPPYAKNPHRVATIADIE
ERGRRLFDPLKRPSDNALVFYRAPTVASVPKAATATATPGRSTAGRRPRTSGKPRPPRQWRPGAVVPPYA
KNPHRVATVADIEQRARMLFDPLKRHADKALVFKRASIKALTVNKQASTVASYFTRQAQHNQIAPAHRRA
IRRIDQQYFALRRAVFSDQRLTRQDKAQLVSVLTFERIKAREQFHKPQPNTEVNFMGSAAIRNLLDDEKE
DPGFSISGARGPGPEGVRDRVKRVMDRFAKQVDPAAASQRARDLSAKDLYTRKAKFSQNVHYLDKQTDKT
LFVDTGKTISMRRTGITEAGVSVALQLARERFGSTLTITGTAEFKKLVIEAVAKNGLDVHFTDKAMNQSL
AARRAELDIERGGQSIGPATDLPRHVDDSTRDVRDQADRMGVTVPIEALYGEGKTAEQVSQALATQLDTV
PEPERIAFVETVAITLGIPERCQPKGDQAFAQWQAQRAQPAADSAATATSEAQTRVATPSDAAPEPVSPT
ASTNPAPASPEAAKPALANDPDLQSPSELVRLEAQWRRDFPMSEADVRASDTVMGLRGEDHAVWIIATND
KTPEAAAMLASYMENDSYREAFKASIVAAYKQVENSPKLVDDLDHLTAMAAQIVNEVEERLFPTPQAATG
QTAPSRSKVIEGTLIEHGEAPYQHNDDNQMSYFVTLKPEGGKPRTVWGVGLEEAMSDAGLKQGDQVRLQD
LGTQPVVVQVIEEDGTVTDKTVNRREWSAQPVAPEREVAETTPKGQAAAAGTPELSSPDEEDGMSMD

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(757-842)


  Protein structure


Source ID Structure
AlphaFold DB H1ZX26

  Reference


[1] Maite Añorga et al. (2022) Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335. Frontiers in microbiology. 13:1076710. [PMID:36578579]


Host bacterium

ID   1487 GenBank   NC_019266
Plasmid name   pPsv48B Incompatibility group   -
Plasmid size   45220 bp Coordinate of oriT [Strand]   37837..38057 [+]
Host baterium   Pseudomonas savastanoi pv. savastanoi

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -