Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100066 |
| Name | oriT_pBSDMV46A  |
| Organism | Bhargavaea cecembensis |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NC_019270 (_) |
| oriT length | 35 nt |
| IRs (inverted repeats) | 1..6, 10..15 (CCAGAA..TTCTGG) |
| Location of nic site | 24..25 |
| Conserved sequence flanking the nic site |
TACCGTG|TTAT |
| Note | _ |
oriT sequence
Download Length: 35 nt
>oriT_pBSDMV46A
CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] You Y et al. (2012) Detection of a common and persistent tet(L)-carrying plasmid in chicken-waste-impacted farm soil. Appl Environ Microbiol. 78(9):3203-13. [PMID:22389375]
Relaxase
| ID | 62 | GenBank | YP_006961697 |
| Name | Mob_pBSDMV46A |
UniProt ID | H9B4L1 |
| Length | 439 a.a. | PDB ID | |
| Note | nicks plasmid at oriT to initiate conjugation | ||
Relaxase protein sequence
Download Length: 439 a.a. Molecular weight: 52396.34 Da Isoelectric Point: 6.3441
>YP_006961697.1 relaxase (plasmid) [Bhargavaea cecembensis]
MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDERLSEVYCMNR
KDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSANVHNDETTPHMHFDFVPVVWD
EKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQEGILNDKTIGIENIHSLKKYSAEIEQQKEEM
ANEFKSFKYPQKVLKSIEREVEKKTVGVFNKKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEI
KNLKVNVKNFEEDNEHLRYRNIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLH
NLENDRMPKNKEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL
SQQRQQQKKTKSRSSGMEL
MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDERLSEVYCMNR
KDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSANVHNDETTPHMHFDFVPVVWD
EKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQEGILNDKTIGIENIHSLKKYSAEIEQQKEEM
ANEFKSFKYPQKVLKSIEREVEKKTVGVFNKKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEI
KNLKVNVKNFEEDNEHLRYRNIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLH
NLENDRMPKNKEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL
SQQRQQQKKTKSRSSGMEL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | H9B4L1 |
Reference
[1] You Y et al. (2012) Detection of a common and persistent tet(L)-carrying plasmid in chicken-waste-impacted farm soil. Appl Environ Microbiol. 78(9):3203-13. [PMID:22389375]
Host bacterium
| ID | 62 | GenBank | NC_019270 |
| Plasmid name | pBSDMV46A | Incompatibility group | - |
| Plasmid size | 5031 bp | Coordinate of oriT [Strand] | |
| Host baterium | Bhargavaea cecembensis |
Cargo genes
| Drug resistance gene | resistance to tetracycline |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |