Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100065
Name   oriT_pPsv48C experimental
Organism   Pseudomonas savastanoi pv. savastanoi
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_019292 (21047..21266 [+], 220 nt)
oriT length   220 nt
IRs (inverted repeats)      IR1: 77..86, 95..104  (TACAAAAAAG..CTTTTTCGTA)
  IR2: 119..126, 130..137  (CAAAGGGG..CCCCTTTG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   

  oriT sequence  


Download         Length: 220 nt

>oriT_pPsv48C
TAGGTGCAGTGTGCACTTGTCTTTTGGAACCGTACTCGAAATCCGAATTCGCTAAACAGGCACGTTACCGATTGTCTACAAAAAAGAGCTTCGCCTTTTTCGTATCCAATCGACGTGCCAAAGGGGATACCCCTTTGGAACCCCGCAGAGCGGGAAAGCGTTGTTGTTGTCGTTGTTGGTTGTCGTCATTCCAGGTGCCCCCAGGAGGTGATCTTGTGGC

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Maite Añorga et al. (2022) Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335. Frontiers in microbiology. 13:1076710. [PMID:36578579]


Relaxase

ID   1083 GenBank   CBZ40064
Name   MOBPpPsv48C experimental UniProt ID   H1ZX53
Length   1254 a.a. PDB ID   
Note   

  Relaxase protein sequence


Download         Length: 1254 a.a.        Molecular weight: 138638.23 Da        Isoelectric Point: 9.5211

>CBZ40064.1 putative relaxase, mobilization protein (plasmid) [Pseudomonas savastanoi pv. savastanoi]
MLIRVSGYNTGAQEYLEKGNKSGREFTRDELDHRLIIEGQLSLTRAIYESIPDYGQDRYLTFALSFKEDI
VSPELLKAVTNDVKNFLMHAYKPEEFNFYAEAHLPKMKTITDRKTDEVIDRKPHIHIIIPRINLLSGNEA
NPVDVYKNHEKYFEAIQEHINQKYGLSSPRENVRADITDAASVLSRYKGDDFYGKNRQFKQELVKQVIER
GVTSRADFYALVAEHGETRIRNQGKDTEYISVKLPGDAKGTNLKDTIFQDDFIVRRELKKPPLEASVIQE
RLLAWPQRAREIKYVNKATPKFRKQYSEASPEDRVRLLADREAKFYQVHGEHNDNVHNGQRQRDHQRSSA
ETAGRRTSAPADGLQDLSVSHVADHRQAGSARSRDGAVLLPSDAHVHVGQSQPGGDSGLRPSVPGGGRGR
RNGSTTERGRGGSGPAAVSQEATGTATPAGATGRRRTGAGKPRNARVRAGRIIPPYAKNPHRVATIADIE
ERGRRLFDPLKKPSDNALVFYRAPSNTPATATTTSGRSSAGRKPRTSGKPRPPRQWRPGSVVPPYAKNPH
RVATVADIEQRARMLFDPLKRPADKALVFKRASIKALTVNRQASTVAAYFTREAQHNQIAPAHRRAIRRI
DQQYFALRRAVFSDQRLTRQDKAQLVSVLTFERLKAREQFHNPQPKTEVNFMGSAAIRNLLDDEKEDPGF
SISGARGPGPEGVRDRVKRVMDRFAKQVDPVAASERARDLSAKDLYTRKAKFSQNVHYLDKQTDKTLFVD
TGTTISMRRTGITEAGVSVALQLARERFGSTLTINGTAEFKKLVIEAVAKNGLDVHFTDKAMNQSLADRR
AELDIERDGQSIGPATDLPRHVDDATRDVRNQADRLSVTVPIEALYGEGKTAEQVSLALATQLDTVPEPE
RIAFVELVAITLGIPERGEPKGDQAFAQWQAQRAQPAADSTATATSEAQANVPTASDTAPEAVTPTAATS
PASSASPDAAKPARLNDPDLQSPSELVRLEAQWRRDFPMSEADVRASDTVMGLRGEDHAVWIIATNDKTP
EAAAMLTAYMENDSYREAFKASIVAAYKQVENSPKLVDDLDHLTAMAAQIVNEVEDRLYPEPQSASAQAT
PPRSKVIEGTLIEHGEAPYQHKDTNQLSYFVTLKPEGGKPRTVWGVGLEEAMTDANLKQGNQVRLEDLGT
WPVVVQVIAEDGTTSDKKVNRRAWSAQPVAPEREVAETTPKGQAAAAGTPELSSPDEEDGMSMD

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(40-255)

Large polyvalent protein-associated domain 7 (LPD7)

(753-838)


  Protein structure


Source ID Structure
AlphaFold DB H1ZX53

  Reference


[1] Maite Añorga et al. (2022) Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335. Frontiers in microbiology. 13:1076710. [PMID:36578579]

ID   1084 GenBank   CBZ40082
Name   MOBQpPsv48C experimental UniProt ID   H1ZX69
Length   705 a.a. PDB ID   
Note   

  Relaxase protein sequence


Download         Length: 705 a.a.        Molecular weight: 77410.93 Da        Isoelectric Point: 7.5352

>CBZ40082.1 putative mobilization protein, MobA/MobL superfamily (plasmid) [Pseudomonas savastanoi pv. savastanoi]
MRTGLVHDYTRRGGVVSTEIMLPDGTSAERNALWNAAESAEKRKDGRTGREWIIALPAELDDGARQELAS
AFGIELATRYGVAVDLAIHLPDREGDNRNHHAHVMTTTRQVSRDAAGLLVMGEKSTIELSDTKRRGMGLG
SGADEVVAIRQLWERMANRALENAGSDARIDSRSLKAQGLDREATTHLGPVASDMERRNKASNRGDGNRQ
VAANNALREQLSAQILDLNAFRAAREAAPVTPAHKTHSAPVRLPDNPLPVYARNPRKMATLDDISMRGAR
LFGPLKAAAEKDQANESGADLLRPEVKAAWNARRAPGTAAVTAAEPEVNATDSVRTHWNPRTTIAPQPAP
VPALPEETPASQAIDAPASSETPKPTERPRPNARPAIEALFAEWRQERGTEKPVAAEAPANIDPQPLSYR
ERMDAIKARRALRQSDQPPVAPDAAQRTPEPIKPVIDPQPQLTGSAAMRARLGVDESRNVDPRGVRERMH
AHVSGQPLLPKVEPAPQPKPEPVKAAPVVPAPEPAKPVISAARIELDTLKASLFSVDERVEADPRVIEAT
RKLNKVVAESQHQIALGPEAARKAAQWRAEHPFRATLHDRGIKNSPELREAERLVSVAALAKDNSHHWES
DARYHLDREKNRVRDGLWDERRAIEKRIEALEPILAAEDALEHPEPSQQARARPPQRSERIYRPDTDIDE
PGMDF

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(2-198)


  Protein structure


Source ID Structure
AlphaFold DB H1ZX69

  Reference


[1] Maite Añorga et al. (2022) Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335. Frontiers in microbiology. 13:1076710. [PMID:36578579]


Host bacterium

ID   1488 GenBank   NC_019292
Plasmid name   pPsv48C Incompatibility group   -
Plasmid size   42104 bp Coordinate of oriT [Strand]   21047..21266 [+]
Host baterium   Pseudomonas savastanoi pv. savastanoi

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -