Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100063 |
| Name | oriT_pBHS24  |
| Organism | Bacillus sp. 24 |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NC_019230 (3425..3459 [+], 35 nt) |
| oriT length | 35 nt |
| IRs (inverted repeats) | 1..6, 10..15 (CCAGAA..TTCTGG) |
| Location of nic site | 24..25 |
| Conserved sequence flanking the nic site |
TACCGTG|TTAT |
| Note | _ |
oriT sequence
Download Length: 35 nt
>oriT_pBHS24
CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Phelan RW et al. (2011) Tetracycline resistance-encoding plasmid from Bacillus sp. strain #24, isolated from the marine sponge Haliclona simulans. Appl Environ Microbiol. 77(1):327-9. [PMID:21057017]
Relaxase
| ID | 59 | GenBank | YP_006960887 |
| Name | Mob_pBHS24 |
UniProt ID | E2G6N9 |
| Length | 439 a.a. | PDB ID | |
| Note | nicks plasmid at oriT to initiate conjugation | ||
Relaxase protein sequence
Download Length: 439 a.a. Molecular weight: 52396.34 Da Isoelectric Point: 6.3441
>YP_006960887.1 Mob (plasmid) [Bacillus sp. 24]
MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDERLSEVYCMNR
KDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSANVHNDETTPHMHFDFVPVVWD
EKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQEGILNDKTIGIENIHSLKKYSAEIEQQKEEM
ANEFKSFKYPQKVLKSIEREVEKKTVGVFNKKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEI
KNLKVNVKNFEEDNEHLRYRNIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLH
NLENDRMPKNKEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL
SQQRQQQKKTKSRSSGMEL
MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDERLSEVYCMNR
KDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSANVHNDETTPHMHFDFVPVVWD
EKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQEGILNDKTIGIENIHSLKKYSAEIEQQKEEM
ANEFKSFKYPQKVLKSIEREVEKKTVGVFNKKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEI
KNLKVNVKNFEEDNEHLRYRNIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLH
NLENDRMPKNKEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL
SQQRQQQKKTKSRSSGMEL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | E2G6N9 |
Reference
[1] Phelan RW et al. (2011) Tetracycline resistance-encoding plasmid from Bacillus sp. strain #24, isolated from the marine sponge Haliclona simulans. Appl Environ Microbiol. 77(1):327-9. [PMID:21057017]
Host bacterium
| ID | 59 | GenBank | NC_019230 |
| Plasmid name | pBHS24 | Incompatibility group | - |
| Plasmid size | 5031 bp | Coordinate of oriT [Strand] | 3425..3459 [+] |
| Host baterium | Bacillus sp. 24 |
Cargo genes
| Drug resistance gene | resistance to tetracycline |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |