Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100056
Name   oriT_pBSDMV9 in_silico
Organism   Bhargavaea cecembensis
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NC_015509 (3400..3434 [+], 35 nt)
oriT length   35 nt
IRs (inverted repeats)      1..6, 10..15  (CCAGAA..TTCTGG)
Location of nic site      24..25
Conserved sequence flanking the
  nic site    		  TACCGTG|TTAT
Note   similar to sequence:RefSeq:NC_014015.1 (plasmid pSU1)

  oriT sequence  


Download         Length: 35 nt

>oriT_pBSDMV9
CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Alippi AM et al. (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]


Relaxase

ID   54 GenBank   YP_004444787
Name   Mob_pBSDMV9 insolico UniProt ID   F5BCQ3
Length   444 a.a. PDB ID   
Note   nicks plasmid at oriT to initiate conjugation

  Relaxase protein sequence


Download         Length: 444 a.a.        Molecular weight: 51819.84 Da        Isoelectric Point: 6.6297

>YP_004444787.1 Mob protein (plasmid) [Bhargavaea cecembensis]
MLFLTENEVSEIAHVQKYTKANMQGLSIHLDRKTENHSNEDIDIERSHLNYDLCEKEGDTLSRMNERLDE
VYCMDRKDVKACCSWVVTLPENLKDAPDEHQRKFFEETYGFLADRYGGEKNVLSANVHNDETTPHMHFAF
IPVVYDPKKEREKVSAKLVINRNELKVFHKDLDVHLKESIPEIYQDGILNDKTIGLDDIESIKKHSKEIE
AKKADMSKELEEFSRKEKEEMVKDLATFKKPDEIIKRIDSTSKESLLGGKTNLITKDYKQLIKVAKSAIK
EKQNLYNYKKVSSEKIGDLEATVRFADRKIKNLEGKNENLEKEVAGLQEHRINEIVFKSMLQDDNRDLNI
SQTEIDGRLILFNLENGHEPLDREQGERWSSTLEENKRLKTIPQSRLQRGLDKLKQFIEKFLVRGFSMDS
VLQKVEQEKSRKPKNKKKSHDLEL

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(11-178)


  Protein structure


Source ID Structure
AlphaFold DB F5BCQ3

  Reference


[1] Alippi AM et al. (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]


Host bacterium

ID   54 GenBank   NC_015509
Plasmid name   pBSDMV9 Incompatibility group   _
Plasmid size   5108 bp Coordinate of oriT [Strand]   3400..3434 [+]
Host baterium   Bhargavaea cecembensis

Cargo genes

Drug resistance gene   resistance to tetracycline
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -