Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100050
Name   oriT_pPL395 in_silico
Organism   Paenibacillus larvae
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   KF440690 (3425..3458 [+], 34 nt)
oriT length   34 nt
IRs (inverted repeats)      1..6, 9..14  (CCAGAA..TTCTGG)
Location of nic site      23..24
Conserved sequence flanking the
  nic site    		  TACCGTG|TTAT
Note   _

  oriT sequence  


Download         Length: 34 nt

>oriT_pPL395
CCAGAAGATTCTGGTATACCGTGTTATACCAAAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Alippi AM et al. (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]


Relaxase

ID   48 GenBank   AGX24954
Name   Mob_pPL395 insolico UniProt ID   U5LK84
Length   439 a.a. PDB ID   
Note   nicks plasmid at oriT to initiate conjugation

  Relaxase protein sequence


Download         Length: 439 a.a.        Molecular weight: 52396.34 Da        Isoelectric Point: 6.3441

>AGX24954.1 Mob (plasmid) [Paenibacillus larvae]
MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDERLSEVYCMNR
KDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSANVHNDETTPHMHFDFVPVVWD
EKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQEGILNDKTIGIENIHSLKKYSAEIEQQKEEM
ANEFKSFKYPQKVLKSIEREVEKKTVGVFNKKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEI
KNLKVNVKNFEEDNEHLRYRNIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLH
NLENDRMPKNKEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL
SQQRQQQKKTKSRSSGMEL

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-173)


  Protein structure


Source ID Structure
AlphaFold DB U5LK84


Host bacterium

ID   48 GenBank   KF440690
Plasmid name   pPL395 Incompatibility group   _
Plasmid size   5030 bp Coordinate of oriT [Strand]   3425..3458 [+]
Host baterium   Paenibacillus larvae

Cargo genes

Drug resistance gene   resistance to tetracycline
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -