Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100049 |
| Name | oriT_pPL374  |
| Organism | Paenibacillus larvae |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | KF536616 (3424..3457 [+], 34 nt) |
| oriT length | 34 nt |
| IRs (inverted repeats) | 1..6, 9..14 (CCAGAA..TTCTGG) |
| Location of nic site | 23..24 |
| Conserved sequence flanking the nic site |
TACCGTG|TTAT |
| Note | _ |
oriT sequence
Download Length: 34 nt
>oriT_pPL374
CCAGAAGATTCTGGTATACCGTGTTATACCAAAT
CCAGAAGATTCTGGTATACCGTGTTATACCAAAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Alippi AM et al. (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]
Relaxase
| ID | 47 | GenBank | AGX24957 |
| Name | Mob_pPL374 |
UniProt ID | U5LK84 |
| Length | 439 a.a. | PDB ID | |
| Note | nicks plasmid at oriT to initiate conjugation | ||
Relaxase protein sequence
Download Length: 439 a.a. Molecular weight: 52396.34 Da Isoelectric Point: 6.3441
>AGX24957.2 Mob (plasmid) [Paenibacillus larvae]
MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDERLSEVYCMNR
KDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSANVHNDETTPHMHFDFVPVVWD
EKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQEGILNDKTIGIENIHSLKKYSAEIEQQKEEM
ANEFKSFKYPQKVLKSIEREVEKKTVGVFNKKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEI
KNLKVNVKNFEEDNEHLRYRNIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLH
NLENDRMPKNKEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL
SQQRQQQKKTKSRSSGMEL
MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDERLSEVYCMNR
KDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSANVHNDETTPHMHFDFVPVVWD
EKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQEGILNDKTIGIENIHSLKKYSAEIEQQKEEM
ANEFKSFKYPQKVLKSIEREVEKKTVGVFNKKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEI
KNLKVNVKNFEEDNEHLRYRNIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLH
NLENDRMPKNKEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL
SQQRQQQKKTKSRSSGMEL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | U5LK84 |
Reference
[1] Alippi AM et al. (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]
Host bacterium
| ID | 47 | GenBank | KF536616 |
| Plasmid name | pPL374 | Incompatibility group | _ |
| Plasmid size | 5026 bp | Coordinate of oriT [Strand] | 3424..3457 [+] |
| Host baterium | Paenibacillus larvae |
Cargo genes
| Drug resistance gene | resistance to tetracycline |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |