Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100047
Name   oriT_pGS18 in_silico
Organism   Geobacillus stearothermophilus
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NC_010420 (50079..50112 [+], 34 nt)
oriT length   34 nt
IRs (inverted repeats)      4..18, 22..34  (CCCATTTATACCCCT..AGGGGTAAATGGG)
Location of nic site      2..3nt
Conserved sequence flanking the
  nic site    		  _
Note   _

  oriT sequence  


Download         Length: 34 nt

>oriT_pGS18
GTGCCCATTTATACCCCTTTCAGGGGTAAATGGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Stuknyte M et al. (2008) Complete nucleotide sequence of pGS18, a 62.8-kb plasmid from Geobacillus stearothermophilus strain 18. Extremophiles. 12(3):415-29. [PMID:18305896]


Relaxase

ID   45 GenBank   YP_001716052
Name   ORF55_pGS18 insolico UniProt ID   B1GSL9
Length   643 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 643 a.a.        Molecular weight: 76066.48 Da        Isoelectric Point: 9.6081

>YP_001716052.1 TraA protein (plasmid) [Geobacillus stearothermophilus]
MAYFLFRTQIINKADRSAVACAAYRSGEALYSERDGLTKKYGHRDVPPETYILAPKHAPEWVYNRERLWN
EVEKVEKQHNAQLAREIIVALPTQLTNEEQTQLLLDFCKENFADEGMVADIAIHRDKKHNPHAHIMLTMR
PFNEDGTWGKKRKKVNGKSVHLTNWNDKETLLKWRKNFADRINEKFKEKGINDRVSHESYEKQGIDKVPQ
IRLSREAYQYEQRMKREAEKQGKPYEPVTYYGKLNQEIQRINQELKALKQQEKVVSISEYQEEKSLNETI
ESIRKNASLNDVEKASLLLVAKRAKSYVDFAVARRVYHDLAEGNWKKKIDSQQLKLRALKNVINKAYHVY
QQEPRQVIQYGFHPGKFNEQLREKIAQLKEMEKSFENELTKYEAVLKKAELALEVQKRLTEEEFRVLYPH
AGADFRIEEKYHAVQYFKDTGSVLPESEIPAYAAKKEQEIHRMPTFAEQTRNMYQSLFVLNRTIQKQSKE
RMEALKVRDFEQAFEASRKIEQCMLRKEKMSKELEENKAVLQAILSEHYGRDVSITNTAALLRLHELVER
GESSKDLEADLRRIHVSEQQKLERMPKPETEAQIMEYQYRQHVADGLLEAIEEAQRANDRKKYEKDPTKK
RQRRRYRGQEFER

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(16-223)


  Protein structure


Source ID Structure
AlphaFold DB B1GSL9

  Reference


[1] Stuknyte M et al. (2008) Complete nucleotide sequence of pGS18, a 62.8-kb plasmid from Geobacillus stearothermophilus strain 18. Extremophiles. 12(3):415-29. [PMID:18305896]


T4CP

ID   45 GenBank   YP_001716053
Name   TrsK_pGS18 insolico UniProt ID   B1GUT7
Length   711 a.a. PDB ID   _
Note   Type IV secretory system Conjugative DNA transfer

  T4CP protein sequence


Download         Length: 711 a.a.        Molecular weight: 81267.80 Da        Isoelectric Point: 4.2876

>YP_001716053.1 TrsK protein (plasmid) [Geobacillus stearothermophilus]
MNLKKDAWKPIFASVVLTVIAAYVLMGLFYMIGNGGDLKEILLRPKELITFVYTEENLKTVFSLAPFVIL
AALLYTFRSSIIVPKLEYASDFGLHGTSRWGTPDEVIDGKMFSKNNSYTTDPVDAFQMEKGIIVGKVPKK
RELLIIPRNTTIDNRNVLVIGSSGSGKGQSFVFPNMINHTEETIIVTDPKGEIYEATHQIKRDQGYKVYQ
VDFINFVNCDRYNPLDYVEDDEDARAIANTIASNSVDEGKRDFWSESAVAFLSAFILYVKEKYKDKANMR
HVVEMVARAGKDEEYLDEIIKNMSPDHPAYHMFTLANMSSGNTRAGIMATLAQQISIFAMQKIAKMTAKS
DFKFHDLQKEKSVLYIKIRMDENPFVQLTATFFEQLIAVLYDIADKNHSKLPIPTIFLLDEFANIGTINK
YPRVLATCRGLGMAMMTIIQDIGQLEDKKRYGPEMARSIINNHDTQLFLRTKDTKTAKYFSELAGETTVK
HKQKSASYGRKEGSKSISEQYVKRSLITPGELMNMDKNTCYLFVSGAYPMKLEKAWQFDIYGDLLSNYEK
YRSRLGYTAPLWNGEWVWDEEDSAAQDIQEVKQELEESDVVLLNEMIDEINKIDEDEEDEEITEEELRVL
LGNVEDENEDEENESEEVGEAEELQSILDEIEEELDAFDEMEDEITEEDLMEIIEEFSEFSDDSEGEQEE
SNQKIADELPI

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(127-550)

  Protein structure



No available structure.



  Reference


[1] Stuknyte M et al. (2008) Complete nucleotide sequence of pGS18, a 62.8-kb plasmid from Geobacillus stearothermophilus strain 18. Extremophiles. 12(3):415-29. [PMID:18305896]


Host bacterium

ID   45 GenBank   NC_010420
Plasmid name   pGS18 Incompatibility group   _
Plasmid size   62830 bp Coordinate of oriT [Strand]   50079..50112 [+]
Host baterium   Geobacillus stearothermophilus

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -