Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100044
Name   oriT_pTcM1 in_silico
Organism   Acidithiobacillus caldus
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_010600 (_)
oriT length   286 nt
IRs (inverted repeats)      59..63, 69..73  (CTTTC..GAAAG)
  124..129, 133..138  (ACATGC..GCATGT)
Location of nic site      197..198
Conserved sequence flanking the
  nic site    		  CATCCTG|C
Note   _

  oriT sequence  


Download         Length: 286 nt

>oriT_pTcM1
TCCTGTCGTTCCTCCTGTGCCGGTTTTCTTAAGCTTTGCATAGATTCTACTTCATTGACTTTCGTAAAGAAAGCACTACAATACGTATAAGCTATATTTTGAACAGGATGGGACTTGTCATACACATGCTACGCATGTTCCCGCCAAGCCACGCCCAACCATTCGGCGCTACGCTTCTCACGGTCCGGCTCATCCTGCAAAGGGCAAGCCCCTTGACCCCGGCCAGCGCGGCGCCATGGCTTCTTGCTGACCCAGACACACCCCGCCGCCGCGAAAGGAGACACGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] van Zyl LJ et al. (2008) Presence of a family of plasmids (29 to 65 kilobases) with a 26-kilobase common region in different strains of the sulfur-oxidizing bacterium Acidithiobacillus caldus. Appl Environ Microbiol. 74(14):4300-8. [PMID:18515486]


Relaxase

ID   42 GenBank   YP_001837312
Name   MobL_pTcM1 insolico UniProt ID   B1A4K1
Length   928 a.a. PDB ID   
Note   relaxase/mobilization nuclease domain-containing protein

  Relaxase protein sequence


Download         Length: 928 a.a.        Molecular weight: 106019.14 Da        Isoelectric Point: 9.8030

>YP_001837312.1 relaxase/mobilization nuclease domain-containing protein (plasmid) [Acidithiobacillus caldus]
MAIYHLHAKTWSKGAGQGAGGHARYVLRQGPYARRVIERVDGSAVRRVEEDRAAEVVASYSGHLPAWADG
DPLAYWDAADAYERANGTVYRELEFALPGELSERRNLKLAQAFAEALARVDRGATPYTLVLHRSTRDPEL
LHCHLMLSDRVNDGYERSPELWFRRAANRGKDPARGGAPKTQARIAQDWLGTVVRPLWAELANAALEQAR
CGARIDHRSLEAQRRERELLAEQAQGRGDELAAARYRKAAGILDRPAQPKRGRVLEHGGPDKAPGQALVW
ARYQEDLARRRAARERLAEADGVVRELREEIARERRREERRAAAKRELDASHGWRPDRVATAQMVLAAVL
DLGQAHREAVQERWDARRERRRVAIEAQRRVEQERQERRGQLLGLAASLAADAECRLRLREAYEAAGFRL
EQRPDGKWVFVYLHGDLLEERQAVREARRAWDERRAALEAQRAAAREAEERRRREGERDRATEQEQGPGV
RHPDKPRWQVERELILTEVYGSAVAEHMGRWYRIERRADALVLSNREASVTDFGDRVVAHEGNDKEIEAL
VLLAQAKGWERVTLTGPREFQERAAQAFVKAGIALEDQALEARAKEAIERQRREDIRERALLRQGYRRDA
WEIRERWHWRQQERARRLEAERELARIEEEAEQARDAWRTVWRKTGHLVDADAQEPIPWGIWREQTLAER
YGPDIAARAEAEYWYTRLRPDLGGLDISRNGQEVVDAGAALQVCSAGSMSLAVDLVRAKGWKRVAIGGPE
DFRERAARALIEAGMVLADEALEQRAKAALDAERVQQERERALRRAMEGIKAHRIRWRRKDGTMGEQRAL
DYLEDRLFRGFTIQETAEGFRWWSADGKPGAVVGDAQLMALFRAAKAADQDLVRVVERIRMEVGMPNAAA
DRARGRTVTPKRGLGDDW

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(523-607)

Large polyvalent protein-associated domain 7 (LPD7)

(726-798)

MobA/MobL family (MobA_MobL)

(63-227)


  Protein structure


Source ID Structure
AlphaFold DB B1A4K1

  Reference


[1] van Zyl LJ et al. (2008) Presence of a family of plasmids (29 to 65 kilobases) with a 26-kilobase common region in different strains of the sulfur-oxidizing bacterium Acidithiobacillus caldus. Appl Environ Microbiol. 74(14):4300-8. [PMID:18515486]


Host bacterium

ID   42 GenBank   NC_010600
Plasmid name   pTcM1 Incompatibility group   _
Plasmid size   65158 bp Coordinate of oriT [Strand]   
Host baterium   Acidithiobacillus caldus

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   arsenic resistance
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -